摘要
目的探讨检测晚发型阿尔茨海默病（LOAD）患者血清中胰高血糖素样肽1（GLP-1）的水平及其临床意义。方法病例对照研究。收集2012年10月至2014年3月期间上海市东方医院和浦东新区部分老年医院诊断为脑血管病（CVD）55例、LOAD61例、2型糖尿病（T2DM）51例、T2DM合并LOAD37例；以及2013年9月至2014年2月上海市东方医院体检中心40名健康体检者作为正常对照（NC）。采用本实验室自建的ELISA方法检测NC、CVD、LOAD、T2DM以及T2DM合并LOAD患者GLP-1的含量，同时测定β淀粉样蛋白（1-42）（β-amyloid，AB）、Tau蛋白以及其他常规生化指标；并测定LOAD组、T2DM组以及T2DM合并LOAD组、NC组的研究对象空腹、服糖后1h、2h三个时间点患者血清中GLP-1的含量。多组间血清GLP-1的浓度比较采用单因素方差分析，两组间血清GLP-1的浓度比较采用LSD—t检验；并采用Pearson相关分析研究GLP-1与其他指标的相关性。结果LOAD组空腹GLP-1水平（123．4±20．8）nmol／L，高于NC组（78．6±6．0）nmol／L和CVD组（89．0±8．7）nmol／L（F值分别为3．46和1．98，P均〈0．05）；T2DM合并LOAD组空腹GLP-1水平（157．9±28．6）nmo]／L高于LOAD组（123．4±20．8）nmol／L（t=1．63，P〈0．05）；T2DM合并LOAD组空腹GLP-1水平（157．9±28．6）nmol／L与T2DM组空腹GLP-1水平（153．8±18．0）nmol／L比较差异无统计学意义（t=0．96，P〉0．05）。服糖后1hT2DM合并LOAD组GLP-1水平（99．1±14．2）nmol／L、LOAD组GLP-1水平（73．9±6．6）nmol／L和T2DM组GLP-1水平（96．3±7．0）nmol／L均出现GLP-1分泌不足情况；服糖后2hT2DM合并LOAD组GLP-1水平为（115．4±18．6）nmol／L，高于正常对照2h水平（63．3±6．2）nmol／L（t=4．49，P〈0．05）；而服糖后2hLOAD组GLP-1水平为（73．6±5．8）nmol／L与正常对照2h水平（63．3±6．2）nmol／L比较差异无统计学意义（t=0．94，P〉0．05）。P
Objective To explore the serum level of Glucagon like peptide-1 in late-onset Alzheimer＇s patients and its clinical significance. Methods Case control study. Collecting cerebral vascular disease fifty-five cases, diagnosed with late-onset Alzheimer＇s disease sixty-one cases, type 2 diabetes mellitus fifty-one cases, type 2 diabetic patients combined with late-onset Alzheimer＇s disease thirty-seven patients from the Shanghai East Hospital and partly Pudong area elderdly hospital during October 2013 to March 2014, and forty healthy persons as normal control from physical examination center of Shanghai East Hospital during September 2013 to February 2014. Measuring the concentrations of GLP-1, β-amyloid, Tau protein and other routinely used clinical tests in the serum of patients from the normal controls, cerebrovascular disease, late-onset Alzheimer＇s disease, type 2 diabetes and type 2 diabetes mellitus combined with late-onset Alzheimer＇s disease by ELISA method developed in our laboratory. The blood samples were also collected at three fixed time including fasting time, 1 hour after taking glucose, 2 hour after taking glucose, the concentrations of GLP-1 were determined in the LOAD group, T2DM group and the T2DM combined with LOAD group and normal control group. The concentrations of serum GLP-1 among groups were compared with single factor analysis of variance, and the concentrations of serum GLP-1 between the two groups were compared using LSD-t test. Analysing the correlation between GLP-1 and other indicators with Pearson analysis. Results The fasting GLP-1 levels of LOAD group were （ 123.4 ± 20. 8 ） nmol/L, and they were highest between the normal control group （78.6 ± 6. 0）nmol/L and the cerebral blood vessel disease group（89.0± 8.7） nmol/L （ F values were 3.46 and 1.98, P 〈 0. 05）. The fasting GLP-1 levels of T2DM combined with LOAD group （ 157.9 ± 28. 6） nmol/L were higher than the LOAD group（ 123.4 ± 20. 8） nmol/L （ t = 1.63, P 〈 0. 05 ）, but there were no
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2015年第8期543-547,共5页
Chinese Journal of Laboratory Medicine
基金
上海市科委生物医药领域重点项目（11DZl973802）
