摘要
目的:研究吉西他滨热化疗灌注联合卡铂化疗栓塞对血清恶性生物学指标以及肝癌组织中凋亡调控基因表达的影响。方法:将本院收治的70例原发性肝癌患者纳入研究,随机分为两组,观察组接受吉西他滨热化疗灌注联合卡铂化疗栓塞治疗,对照组接受吉西他滨常规灌注化疗联合栓塞治疗,比较两组血清恶性生物学指标以及肝癌组织中凋亡调控基因的表达。结果:(1)血清恶性生物学指标:观察组血清DKK1、TK1、HIF-1α的mRNA和蛋白含量低于对照组,差异均具有统计学意义(P<0.05);(2)促凋亡基因:观察组肝癌组织中MTS1、Caspase-3和Bax的mRNA和蛋白含量高于对照组,差异均具有统计学意义(P<0.05);(3)凋亡抑制基因:观察组肝癌组织中Plk1、Bcl-2和Survivn的mRNA和蛋白含量高于对照组,差异均具有统计学意义(P<0.05)。结论:吉西他滨热化疗灌注联合卡铂化疗栓塞有助于抑制肿瘤恶性生物学行为、诱导肝癌细胞发生凋亡,是治疗原发性肝癌的理想方法。
Objective： To study the effects of Gemcitabine heat perfusion chemotherapy combined with carboplatin chemotherapy embolization on serum indexes in patients with hepatocellular carcinoma. Methods： 90 cases of hepatocellular carcinoma patients were enrolled and randomly divided into two groups. Observation group received gemcitabine heat perfusion chemotherapy combined with carboplatin chemotherapy embolization, control group received gemcitabine conventional perfu- sion chemotherapy combined with carboplatin chemotherapy embolization. Malignant biological indicators of serum and liver tissue apoptosis regulation of gene expression of the two groups were compared. Results.. （1） Serum malignant biological indi- cators： serum DKK1, TK1, HIF- 1 alpha mRNA and protein content of the observation group were lower than that of the control group~ （2） Promoting apoptosis gene： MTS1 in liver tissue, Caspase 3 and Bax mRNA and protein contents of the ob- servation group was higher than that of the control group; （3） Apoptosis suppressor genes： liver cancer tissues Plkl, Bcl - 2 and Survivn mRNA and protein contents of the observation group was higher than that of the control group. Conclusion： Gem- citabine hot perfusion chemotherapy plus earboplatin chemotherapy embolism helps to inhibit tumor biological behavior, induce liver cancer ceils apoptosis, and it is an ideal treatment for primary liver cancer.
出处
《海南医学院学报》
CAS
2015年第8期1112-1115,共4页
Journal of Hainan Medical University
基金
四川省卫生厅(080133)~~
关键词
原发性肝癌
热化疗灌注
恶性生物学行为
凋亡
Primary liver cancer
Hot perfusion chemotherapy
Malignant biological behavior
Apoptosis
