摘要
目的研究miR-146a在结核病患者外周血单个核细胞中的表达水平和临床意义。方法采用Trizonl一步法从结核病患者、结核病潜伏感染者和健康对照外周血单个核细胞提取总RNA,荧光定量PCR测定miR-146a的表达。同时使用荧光定量PCR测定样本中肿瘤坏死因子受体相关因子6、白细胞介素受体相关激酶1和肿瘤坏死因子的表达。结果肺结核患者外周血单核细胞miR-146a表达较结核潜伏感染和健康对照者显著下降,而潜伏感染者miR-146a表达水平(4.49±0.61)与对照组(4.57±0.25)没有差异。同时,miR-146a下游靶基因TRAF6、IRAK-1以及TNF-α表达在活动性肺结核患者中较结核潜伏感染和健康对照者增加。结论与健康对照和潜伏感染者相比,miR-146a在结核病患者外周血单个核细胞中表达显著下降,推测miR-146a有可能成为鉴别活动性结核和结核潜伏感染新的标记物。
Objective To study the expression of miR-146a in tuberculosis （TB） patients and latent tuberculosis infection （LTBI）. Methods Total RNA was isolated from peripheral blood mononuclear cells （PBMCs） obtained from patients with TB, and healthy and LTBI individuals, and the expression of miR-146a was analyzed by quantitative real-time PCR. In addition, two targets of miR-146a, namely tumor necrosis factor receptor-associated factor 6 （TRAF6） and IL-1 receptor-associated kinase 1 （IRAK-1） and tumor necrosis factor-α（TNF-α） were also analyzed by quantitative real-time PCR. Results PBMCs of patients with TB exhibited signiifcantly decreased in miR-146a expression, whereas miR-146a expression was not significantly different compared with healthy control individuals （4.57 ± 0.25） and LTBI （4.49 ± 0.61）. TRAF6, IRAK-1 and TNF-α were increased expression between patients with TB and LTBI and control individuals, implicating that miR-146a plays a key role in the active tuberculosis. Conclusions Compare with health control and LTBI, the miR-146a expression level was down-regulated in TB patients. This indicated that miR-146a may be a potentially useful as a biomarker for diagnosis of TB and LTBI.
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2015年第4期56-59,共4页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
"十二五"重大专项子课题(No.2013ZX10003003)
首都医科大学基础临床合作研究项目(No.2014-1-156)
