摘要
针对中棉所49双亲中棉所35和中51504分别含有野生棉种质和海岛棉种质,难以稳定纯化、柱头高于雄蕊,容易产生异交退化等问题,研发了大群体自交纯化提高技术。经过2001~2003年连续3 a的大面积（1.33 hm2/a）全生育期纯度鉴定和自交纯化后入选7个优系,2005~2006年将这7个优系与中棉所49原种（对照）进行品种比较试验,最终确定丰产性、稳定一致性、纤维品质和抗病性综合表现最突出的品系中棉所49-6作为基础种繁育。此后,每年苗期进行2次硫酸卡那霉素鉴定、中期进行表型筛选和自交,2009年以后又增加了开花前DNA分子检测,使中棉所49标准样品位点纯合率达到了100%,商品种子分子纯度达95.8%,成为目前国内高纯度的棉花品种之一。
The technology here elaborated could stabilize and purify CCRI49 easily, which was derived from the cross CRI35 × CRI51504, containing the genetic material of wild cotton and island cotton, and whose stigma standing higher than the stamen. We identified the purity and purified by self-crossing of lines in the area of 20 acres in the three consecutive years from 2001 to 2003, 7 lines were selected to compared with the control CCRI49 and CCRI49-6 was considered to be the accredited because of its high yield potential, excellent stability and conformity, fiber quality and high disease resistance. We identified the resistance of Kanamycin in the seedling stage, evaluated the phenotype and self-crossed in the growing stage since then, and added molecular marker detection before flowering from 2009. The ratio of homozygous DNA locus ratio was 100% in the standard reference materials, and 95.8% in the commercial seeds, being one of high purity varieties in our country.
出处
《河北农业科学》
2015年第4期77-81,共5页
Journal of Hebei Agricultural Sciences
基金
河南省现代农业产业技术体系项目(S2013-07-1)
中央级公益性科研院所基本科研业务费专项资金(1610162014002)
关键词
棉花
大群体自交
基础种筛选
分子标记检测
Cotton
Large group inbred
Basic germplasm screening
Molecular marker detection
