摘要
采用单因素试验方法研究富氮及限氮两种培养体系下甘油浓度(41、110、206mmol/L)对湛江等鞭金藻生长及细胞内多糖累积的影响。试验结果显示,当甘油浓度为41mmol/L时湛江等鞭金藻的细胞密度、细胞内多糖含量及产量均比其他两种甘油浓度时的值高,其中富氮培养体系的细胞密度、细胞内多糖含量及产量分别为7.73×107个/mL、16.21%和0.32g/L,限氮培养体系的细胞密度、细胞内多糖含量及产量依次为5.29×107个/mL、54.43%和1.02g/L。表明培养体系中甘油浓度低而氮含量高时有利于细胞生长(前者是后者的1.46倍),而甘油和氮含量均少时利于细胞积累多糖(后者的多糖含量和产量分别是前者的3.36倍和3.19倍)。在两种培养体系下,随着甘油浓度的增加,湛江等鞭金藻的细胞密度及藻细胞内累积的多糖呈降低趋势,说明高浓度甘油抑制湛江等鞭金藻细胞生长和细胞内多糖的累积。因此,为提高湛江等鞭金藻细胞内的多糖产量,应在接种时添加低浓度甘油的限氮培养体系中培养湛江等鞭金藻,这些结果可为高值天然产物的生产提供参考。
Effects of glycerol concentration （41 mmol/L, 110 mmol/L and 206 mmol/L） on growth and polysaecharides accumulation of alga Isochrysis zhanjiangensis were investigated in rich--nitrogen and limited--nitrogen culture systems by means of single factor experiments. The results showed that the cell density, content and yield of polysaccharides were higher in 41mmol/L glycerol than those in the other glycerol concentrations. The cell density, content and yield of polysaccharides in rich-nitrogen culture system were 7. 73 × 107 cells/mL, 16. 21% and 0. 32 g/L, respectively, while those in limited nitrogen culture system were 5.29× 107 cells/mL, 54.43 % and 1.02 g/L, respectively. These data explained that lower glycerol concentration and higher nitrogen concentration were advantageous to the growth of I. zhanjiangensis （the biomass in the former was 1.46 times as that in the later）, however, when glycerol concentration and nitrogen concentration were lower at the same time, the cell accumulated more polysaccharides （the content and the yield of polysaccharides of the later were 3.36 times and 3.19 times as the former, respectively）. No matter the difference in nitrogen supply, with the increasing concentration of glycerol, the cell density and polysaccharides content of I. zhanjiang-ensis showed a decreasing trend. It indicated that high concentration of glycerol inhibited the growth of I. zhangjiangensis and the accumulation of polysaccharides. So, I. zhangjiangensis should be cultured in the limited-nitrogen culture system with low concentration of glycerol at inoculation to promote the accumulation of polysaccharides. The findings will provide reference with producing natural products of high value.
出处
《水产科学》
CAS
CSCD
北大核心
2015年第8期485-490,共6页
Fisheries Science
基金
国家"863"高技术研究发展计划项目(NO.2012AA052101)
辽宁省自然科学基金资助项目(NO.2012010263)
关键词
湛江等鞭金藻
生长
甘油
多糖
Isochrysis zhangjiangensis
growth
glycerol
polysaccharide
