摘要
以"丰花月季"品种为试材,采用正交实验设计,研究蔗糖、硼酸、CaCl2、MgSO4、ZnSO45个因素不同浓度对其花粉生活力的影响,以期找出最适宜"丰花月季"花粉萌发的培养条件。结果表明:蔗糖浓度对"丰花月季"花粉萌发的影响最大,其余依次为硼酸浓度、ZnSO4浓度、CaCl2浓度,MgSO4浓度的影响最小。一定浓度的蔗糖、硼酸、CaCl2对花粉的萌发有较大的促进作用,但超过一定浓度时起抑制作用,而ZnSO4浓度对花粉的萌发率影响是在一定范围内先降低后升高,MgSO4浓度对花粉的萌发影响不太明显。最适宜"丰花月季"花粉萌发的离体条件是200g/L蔗糖+100mg/L硼酸+50mg/L CaCl2+20mg/L MgSO4+40mg/L ZnSO4。
Taking‘Floribunda Roses＇ as experimental materials,the influence of five factors on pollen viability by orthogonal experiment design were discussed,and the five factors were the concentrations of sucrose,boric acid,CaCl2,MgSO4 and ZnSO4,so as to find out the best matching,namely,the optimum culture conditions of‘Floribunda Roses＇ pollen germination.The results showed that the concentration of sucrose had the greatest influence on ‘Floribunda Roses＇ pollen germination,the concentrations of boric acid,ZnSO4 and CaCl2 had greater influence,and that the concentration of MgSO4 had minimal impact.A certain concentrations of sucrose,boric acid and CaCl2 for pollen germination had great role in promoting,but when the factors exceeded a certain concentration,they would have inhibitory effect.The effect of the concentration of ZnSO4 on pollen germination rate was later increased after the firstly decreased within a certain range.The concentration of MgSO4 had little effect on pollen germination.200g/L sucrose＋100mg/L boric acid＋50mg/L CaCl2＋20mg/L MgSO4＋40mg/L ZnSO4 was the best in vitro culture medium for‘Floribunda Roses＇ pollen germination.
出处
《北方园艺》
CAS
北大核心
2015年第16期72-75,共4页
Northern Horticulture
基金
河南省科技厅资助项目(143400410043)
关键词
“丰花月季”
花粉生活力
正交实验
＇ Floribunda Roses＇
pollen germination
the orthogonal experiment method
