摘要
PCR扩增弓形虫棒状体蛋白21(Tg ROP21)全长基因序列,利用Signa IP和TMHMM在线网站预测分析其信号肽、跨膜区;DNAStar软件分析其亲水性、抗原指数;联合运用Ex PASY和PRODATA在线网站对Tg ROP21的功能域及三级结构进行建模。PCR产物可见1条2 022 bp左右的片段,与预期片段大小相同。成功预测出Tg ROP21的信号肽、跨膜区、亲水性、抗原指数、功能域和三级结构。
The full-length gene sequence of Toxoplasma gondii ROP21（TgROP21） gene was amplified with PCR. The signaling peptide and transmembrane domain of TgROP21 protein were predicted by SignaIP and TMHMM online predictive sites, and the hydrophilicity and antigenic index of this protein were ananlyzed with DNAStar software. Meanwhile, the functional domains and tertiary structure were modeled by combined use of ExPASY and PRODATA online sites. As expected, the PCR results revealed one band at 2 022 bp. The signaling peptide, transmembrane domain, hydrophilicity, antigen index, functional domain and 3D structure of TgROP21 were successfully predicted. This work may provide a theoretical foundation for further verification of TgROP21 function.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2015年第4期294-296,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
山东省优秀中青年科学家科研奖励基金(No.BS2013SW015)
山东省医药卫生科技发展计划项目(No.WSO370)
山东省医学科学院科技计划项目(No.2013-47)
济宁市科技计划(医疗卫生)项目(2013wk79)~~
