摘要
目的研究姜黄素对人早幼粒白血病细胞HL-60细胞增殖和凋亡的影响及其可能的作用机制。方法将HL-60细胞设立实验组、阴性对照组、空白对照组,实验组分姜黄素(0、2.5、5.0、10.0、20.0、40.0μmol/L)单独亚组和姜黄素(0、5.0、10.0、20.0μmol/L+30μmol/L GANT61)联合亚组,于作用24、48 h时观察。采用CCK-8法检测HL-60细胞增殖,计算细胞增殖抑制率;并评价体外联合应用药物对细胞毒性作用是否有协同作用;采用AnnexinⅤ-FITC/PI双染检测细胞凋亡率。结果姜黄素单独亚组和姜黄素联合亚组在24、48 h对HL-60细胞的抑制率比较,差异均有统计学意义(P<0.05);培养至24、48 h时,5.0、10.0、20.0μmol/L姜黄素联合亚组分别与5.0、10.0、20.0μmol/L单独亚组对HL-60增殖抑制率比较,差异均有统计学意义(P<0.05);培养至24、48 h时,10.0、20.0μmol/L姜黄素联合亚组与0μmol/L姜黄素联合亚组对HL-60增殖抑制率比较,差异均有统计学意义(P<0.05)。培养至24 h时,5.0μmol/L姜黄素与30μmol/L GANT61对HL-60细胞的增殖抑制率呈拮抗作用,培养至48 h时,呈单纯相加作用;24、48 h时,10.0、20.0μmol/L姜黄素与30μmol/L GANT61联合用药对HL-60细胞的增殖抑制率均呈增强作用。培养至24、48 h时,姜黄素、GANT61和姜黄素+GANT61对HL-60细胞凋亡率比较,差异均有统计学意义(P<0.05);其中,培养至24 h时,10.0、20.0μmol/L姜黄素联合用药分别与10.0、20.0μmol/L姜黄素单独用药对HL-60细胞凋亡率比较,差异均有统计学意义(P<0.05);培养至48 h时,5.0、10.0、20.0μmol/L姜黄素联合用药分别与5.0、10.0、20.0μmol/L姜黄素单独用药对HL-60细胞凋亡率比较,差异均有统计学意义(P<0.05);5.0、10.0、20.0μmol/L姜黄素联合用药与GANT61单独用药对HL-60细胞凋亡率比较,差异均有统计学意义(P<0.05)。结论姜黄素和GANT61联合用药对HL-60细胞增殖具有协同抑制作用,显著促进HL-60细胞凋亡,而且与浓度和�
Objective To investigate the influence of curcumin on the proliferation and apoptosis of human promyelocytic leukemia HL-60 cells and its possible mechanism .Methods We divided the included HL -60 cells into trial group, negative control group and blank control group .The trial group was further divided into single subgroups （0, 2.5, 5.0, 10.0, 20.0, 40.0 μmol/L curcumin ） and combined subgroups （ 5.0, 10.0, 20.0 μmol/L curcumin ＋30 μmol/L GANT61）.Observations were conducted 24 hours and 48 hours after the trial began.CCK -8 method was used to detect the proliferation of HL-60 cells, and the proliferation inhibition rate was calculated .In vitro combined application of medicines was investigated to observe whether synergistic effect exists .AnnexinⅤ-FITC/PI double-stained method was employed to detect thenbsp;apoptosis rate of cells .Results The single subgroup and combined subgroup were significantly different （ P〈0.05 ） in 24 h and 48 h inhibition rates; the 5.0, 10.0 and 20.0μmol/L combined subgroups were significantly different from 5.0, 10.0 and 20.0 μmol/L single subgroups in 24 h and 48 h proliferation inhibition rates; 10.0 and 20.0μmol/L combined subgroups and 0μmol/L combined subgroup were significantly different （P〈0.05） in 24 h and 48 h inhibition rates.Antagonistic effect occurred in the combined subgroup with 5.0 μmol/L curcumin ＋30 μmol/L GANT61 on 24 h proliferation inhabitation rate , and pure additive effect occurred in this group on 48 h proliferation inhabitation rate; potentiation occurred in the combined subgroups with 10.0 or 20.0μmol/L＋30μmol/L GANT61 on 24 h and 48 h proliferation inhabitation rates.Curcumin, GANT61 and curcumin＋GANT61 were significantly different （P〈0.05） in the effect on 24 h and 48 h apoptosis rates; the 10.0 and 20.0 μmol/L combined subgroups were significantly different （P〈0.05） from 10.0 and 20.0μmol/L single subgroups in 24 h apoptosis rate;the 5.0, 10.0 and 20.0μmol/L combined subgroups were significantly dif
出处
《中国全科医学》
CAS
CSCD
北大核心
2015年第23期2800-2804,共5页
Chinese General Practice
基金
国家自然科学基金资助项目(81460024)
石河子大学优秀青年项目(2013ZRKXYQ26)
