摘要
为了解蜜蜂囊状幼虫病毒的遗传变异及分化,采用多聚蛋白基因对其进行遗传结构分析.结果表明:在比对的441bp基因片段中,有356个保守位点,85个变异位点,无缺失位点.构建系统进化树显示该病毒存在3个明显的分支,分支Ⅰ为中国的重庆、云南病毒株;分支Ⅱ为印度病毒株;分支Ⅲ为德国、英国、奥地利、韩国病毒株.中国、韩国、尼泊尔、印度等亚洲地区存在多个病毒株.中介网络图显示病毒株基本按照宿主种类进行聚类,表明同一地区存在多个病毒株系可能与宿主有密切关系.
The genetic structure of Sacbrood bee virus(SBV) has been studied through analyzing polypro- tein gene for acquainting the genetic variation and differentiation of SBV in the experiment. The results show that there were three hundred and fifty--six conservative sites, eighty--five variation sites and no deletion loci in alignment four hundred and forty--one gene fragment, respectively. It also shows that SBV existed three obvious branches through constructing phylogenetic tree. Chongqing and Yunnan virus strains from China are branch I, India virus strains branch II, and Germany, Britain, Austria, and Korea virus strains branch III. There are multiple virus strains in some Asian regions such as China, Korea, Ne- pal and India. Median joining network shows that SBV strains clustered according to host basically. It in- dicates that it may have close relationship with the host for the reason of multiple SBV strains in the same area.
出处
《西南师范大学学报(自然科学版)》
CAS
北大核心
2015年第7期102-107,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
云南省农业科学院优秀团队建设培育及科研超前预研(基础性超前预研研究)项目(YAAS2014JC014)
云南省农业科学院青年创新基金资助项目(QC2012001)
国家现代农业产业技术体系项目(CARS-45-SYZ17)