摘要
目的探讨肺炎支原体肺炎（MPP）患儿外周血IL-10/IL-17表达与肺功能变化的相关性。方法选取66例MPP患儿,依据是否闻及肺部哮鸣音分为MPP喘息组（n=31）和MPP非喘息组（n=35）,选取健康体检儿童30例为对照组。采用ELISA法检测各组IL-10、IL-17水平;采用肺功能检测仪检测呼气高峰流量（PEF）、第1秒时间肺活量（FEV1）、第1秒时间肺活量/用力肺活量（FEV1/FVC）及肺活量最大呼气流速（V75,V50,V25）。结果 MPP喘息组、非喘息组与对照组三组相比较,IL-10及IL-17差异有统计学意义（F=34.788、30.957,P均〈0.05）,其中MPP喘息组和MPP非喘息组血清IL-17水平均高于对照组,差异有统计学意义（P均〈0.05）;MPP喘息组和MPP非喘息组血清IL-10水平均低于对照组,差异有统计学意义（P均〈0.05）。与MPP非喘息组相比较,MPP喘息组PEF、FEV1、FEV1/FVC、V75、V50和V25均明显降低,差异具有统计学意义（t=3.268-5.362,P均〈0.05）。Pearson相关分析显示,MPP患儿血清IL-10表达水平与PEF、FEV1、V75、V50、V25呈正相关（r=0.285-0.492,P〈0.05）;IL-17表达水平与PEF、FEV1、V75、V50、V25呈负相关（r=–0.327-–0.451,P〈0.05）。结论 MPP患儿存在IL-10/IL-17免疫平衡异常现象,且IL-10/IL-17表达与肺功能变化存在相关性。
Objective To evaluate the correlation of peripheral IL-10/IL-17 expression and pulmonary function in children with Mycoplasma pneumoniae pneumonia （MPP）. Methods Children with MPP （n=66） were recruited and divided into MPP gasp groups （n=31） and MPP no-gasp group （n=35） based on whether pulmonary wheezing sound exists. Another 30 healthy children were recruited as control group. ELISA was used to detect the level of interleukin - 6 （IL - 6） and transforming growth factor-β （TGF-β）. The peak expiratory flow （PEF）, forced expiratory volume in 1 s （FEV1）, forced expiratory volume in 1 s/forced vital capacity （FEV1/FVC） and lung capacity maximum expiratory flow （V75, V50, V25） were detected by pulmonary function instrument. Results The serum IL-10 level of MPP gasp groups, MPP no-gasp group and control group were （8.1±1.8） ng/L, （13.3±2.0） ng/L and （18.4±1.7） ng/L, respectively; the serum IL-17 level were （23.8±2.3） ng/L, （16.2±1.5） ng/L and （11.4±1.0）ng/L, respectively; which showed significant differences between each group （P 〈 0.05）. The PEF, FEV1 and FEV1/ FVC of MPP gasp groups were （71.4±5.7）%, （71.8±6.3）% and 72.5±9.6; and those of MPP no-gasp group were （95.3±7.8）%, （96.2±10.5）% and 85.3±4.5; which showed significant differences between the two groups （P 〈 0.05）. The V75, V50 and V25 were （74.7±5.0）%, （45.2±10.3）% and （38.3±8.7）%, and those of MPP no-gasp group were （85.6±4.2）%, （75.9±9.5）% and （66.1±12.5）%. Pearson correlation analysis showed that PEF, FEV1, V75, V50 and V25 had positive correlation（P〈0.05）with the level of IL-10 and f IL-17 （both P〈0.05）. Conclusion MPP children have IL-10/IL-17 imbalance, and the expression of IL-10/ IL-17 was correlated with pulmonary function changes.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2015年第8期686-689,共4页
Journal of Clinical Pediatrics
