摘要
目的探讨血管生成素-1(Ang-1)对糖尿病大鼠肾脏病变的影响。方法 96只雄性SD大鼠随机分为正常对照(NC)组、DN组、Ang-1载体(AV)组和空载体(BV)组。STZ诱导DN模型,8周后尾静脉注射Ang-1-腺病毒载体或腺病毒空载体。检测不同时点血糖、尿蛋白及肾脏病理;采用免疫组织化学、荧光和RT-PCR检测肾组织Ang-1及其受体络氨酸激酶-2(Tie-2)蛋白和mRNA。结果 AV组24hUAlb[8周(52.33±15.67)mg/24h,28周(40.50±7.42)mg/24h]及血糖[8周(26.28±0.81)mmol/L,28周(17.48±1.14)mmol/L]降低(P<0.05);肾脏病理变化减轻,肾小球面积未减小(P>0.05);肾组织Ang-1、Tie-2 mRNA[8周(76.55±13.21),28周(90.47±5.04)]及蛋白[8周(140.85±8.45),28周(150.84±10.48)]升高(P<0.05)。结论外源性Ang-1可上调DN大鼠肾组织Ang-1和Tie-2的表达,缓解DN。
Objective To investigate the effect of recombinant adenovirus angiopoietin‐1（Ang‐1） on renal lesions in diabetic rats. Methods A total of 96 male SD rats were randomly divided into 4 groups ：normal control （NC） group ,diabetic nephropathy （DN） group ,Ang‐1‐vector （AV） group and blank vector （BV） group. The DN model was induced by injection of streptozotocina. Ang‐1‐vector and blank vector were injected by tail intravenous injection 8 weeks later. The blood glucose ,urine protein ,and renal pathology of different time points were detected. Ang‐1 ,Tie‐2 protein and its mRNA in renal tissue were tested by RT‐PCR ,immunochemistry and immunofluorescence respectively. Results The 24 hUAlb[8 weeks（52.33 ± 15.67）mg/24 h ,28 weeks（40.50 ± 7.42）mg/24 h] and blood glucose [8 weeks（26.28 ± 0.81） mmol/L ,28 weeks （17.48 ± 1.14 ） mmol/L ] were decreased significantly （ P 〈 0.05 ） ,renal pathological change was alleviated ,and the decrease of glomerular area in Ang‐1 treated group was not significant （P〉0.05）. The expressional of Ang‐1 and Tie‐2 mRNA [8 weeks （76.55 ± 13.21） ,28 weeks （90.47 ± 5.04）]and protein in renal tissue [8 weeks （140.85 ± 8.45） ,28 weeks（150.84 ± 10.48）]were increased significantly（ P〈0.05）. Conclusion Exogenous Ang‐1 can upregulate the expression of Ang‐1 and Tie‐2 in renal tissue ,and relieve renal lesions in diabetic rats.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2015年第8期747-750,共4页
Chinese Journal of Diabetes
基金
国家自然科学基金(81260118)
贵州省优秀科技教育人才省长专项资金(黔省专合字[2005]242)
遵义医学院博士科研启动基金([2006]13)
