摘要
目的分析中药菊花煎剂对小儿呼吸道合胞病毒(respiratory syncytial virus,RSV)感染气道上皮细胞(A549细胞)后趋化因子的影响。方法以近期苏州大学附属第二医院收治的住院小儿确诊RSV型肺炎患者64例作为研究对象,分析患儿感染RSV后的临床病症。建立RSV感染A549细胞模型,对感染后细胞进行细胞培养,加入中药菊花煎剂和常规抗病毒药观察其对RSV感染细胞的存活率影响;用ELISA试剂盒测定并比较2组细胞液中趋化因子MCP-1及RANTEs的表达情况。结果体外细胞培养实验结果表明,中药菊花煎剂有直接的抑制病毒生长复制的作用,且存在一定的正量效应;RSV感染A549细胞后,MCP-1和RANTEs表达或释放明显增多(P<0.05),中药菊花煎剂及抗病毒药干预后,其表达明显降低(P<0.05),但是其总量仍显著高于正常值。结论中药菊花煎剂能在一定程度上抑制RSV感染A549细胞释放趋化因子RANTEs和MCP-1,从而起到减轻患儿气道炎症反应的作用。
Objective To study effect of traditional Chinese medicine chrysanthemum decoction on RSV infection after A549 cells chemotactic factor.Methods Subjects from The Second Hospital Affilated To Suzhou University in recent days, 64 cases of patients diagnosed with RSV effected pneumonia by clinical examination, RSV infection A549 cell model was established and the infected cells were cultured, chrysanthemums with TCM decoction and conventional antiviral observed its effect on RSV infection cell survival, compared two groups of cell SAP was determined by ELISA kit and the expression of RANTEs and chemokines MCP-1.Results Chrysanthemum, according to the results of in vitro cell culture studies traditional Chinese medicine decoction had the effect of directly inhibit the growth of virus replication, and there were some positive effects RSV infected A549 cells, MCP-1 and RANTEs expression or release significantly increased （ P〈0.05 ） .Chrysanthemum Chinese medicine decoction and antiviral drugs after the intervention, the expression of MCP-1 and RANTES reduced significantly （P〈0.05）, but overall volume was still significantly higher than the normal. Conclusion Chrysanthemum Chinese medicine decoction can inhibit RSV infected A549 cells release chemokine RANTEs and MCP-1 to some extent, and then reduce the airway inflammation reaction of children.
出处
《中国生化药物杂志》
CAS
2015年第3期82-84,共3页
Chinese Journal of Biochemical Pharmaceutics
