摘要
利用蛋白质双向电泳技术和PDQuest分析软件对人工混合的不同纯度大麦的蛋白质电泳图谱进行分析,找到了不同品种大麦间的主要差异蛋白,并应用各差异点的3D高度数值与大麦纯度进行线性拟合,得到了不用差异蛋白质点相关的线性回归方程,并检验其准确性,实验取得了良好的效果,该方法可以较为准确地检测大麦种子的纯度。
In this experiment, different purities of barley cultivars by artificial mixing were analyzed by two-dimensional eleetrophoresis and PDQuest software. The major diverse proteins were found. Linear regression curves of 3D peak？ height values from different protein plot and barley purity were made, and the accuratissime was also detected. The experiment had a good performance. This method can be used to accurately detect the purity of barley cultivars.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第7期185-188,共4页
Food and Fermentation Industries
基金
大连工业大学青年基金(QNJJ201403)资助
关键词
大麦
纯度
二维电泳
barley
purity
two-dimensional electrophoresis
