摘要
目前,针对双酚A(BPA)或镉(Cd)内分泌干扰毒性的研究很多,但是关于其联合作用的研究则较少。将昆明雌性幼鼠分为4组,分别为对照组、BPA组、Cd组及联合组,灌胃染毒5周,观察小鼠动情周期、卵巢组织抗氧化水平、血清性激素及子宫内膜下层α亚型雌激素受体(ERα)免疫组化表达等指标的变化。结果发现,与对照组比较,各染毒组的动情周期均发生变化,体重、子宫系数、血清性激素水平及子宫内膜下层ERα表达降低(P<0.05);BPA组及Cd组的卵巢T-SOD及MDA水平与对照组比较无差异(P>0.05),但联合组的卵巢T-SOD及MDA水平显著区别于对照组(P<0.05);此外,联合组的子宫内膜下层ERα阳性表达率(36%±11.7%)显著低于BPA组及Cd组(P<0.05)。BPA及Cd对雌性生殖内分泌系统联合作用毒性较各单剂量组增加,氧化损伤及影响ERα表达可能是联合毒性作用机制之一。
There are a lot of research about endocrine disrupting toxicity of bisphenol A （BPA） or cadmium（Cd）, but no studies have been carried out to shed light on combined toxicity of BPA and Cd. Forty Kunming female ju- venile mice were randomly divided into 4 groups（normal control, BPA, Cd, and BPA plus Cd group）. The mice were treated by garage for 5 successive weeks. By the end of the experiment, the estrus cycle, antioxidant activity of o- vary, serum sex hormone levels and estrogen receptor-alpha（ER） expression in endometrium of mice were detected. Compared with control group, there are difference on time of estrus, body weight, uterus coefficient, serum E2 lev-els and endometrium ElL expression in treated groups; Ovary T-SOD activity and MDA levels were no signifficant- ly different in BAP group and Cd group compared to the control group （P^0.05）, however, ovary T-SOD activity and MDA levels in collective group were significantly different compared with those of the control group （P〈0.05）. Furthermore, endometrium ElL expression （36± 11.7）% in collective group were lower than those of single- dose groups （P〈0.05）. Combined effect of BPA and Cd is more toxic than that of single dose exposure, impact on EIL expression and oxidative damage may be the mechanisms of the combined toxicological effect.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2015年第3期129-134,共6页
Asian Journal of Ecotoxicology
基金
天津农学院教学改革项目(12-A2-03)
关键词
双酚A
镉
联合作用
雌性
生殖内分泌
bisphenol A
cadmium
combined effect
female
reproductive endocrinology
