摘要
以甜高粱品种能饲一号为野生型构建EMS化学诱变突变体库,通过分析突变体可溶性糖含量、生物质总量和生物质酶解产糖效率,观察农艺性状,并测定秸秆细胞壁结构组成,筛选得到3份特异突变体材料:SM83、SM197、SM305。3份突变体除生物量皆显著提高以外,SM197与SM305半纤维素含量显著升高,而木质素和纤维素含量明显降低,导致生物质酶解产糖效率大大提高。此外,SM83的产糖效率与常规品种(对照)无显著差异,但其可溶性糖含量比野生型增加14.99%。故筛选到的甜高粱突变体是理想的生物能源种质材料,可用于优质能源甜高粱品种的培育。
The large EMS mutagenesis pool of sweet sorghum was initially screened out.Three mutants were identified and designated as SM83,SM197 and SM305.In general,three mutants had high biomass productions,but displayed a distinct biomass enzymatic digestibility under chemical pretreatments.As a comparison,both SM197 and SM305mutants were determined with much high biomass enzymatic saccharification,due to its high hemicelluloses levels and relatively low cellulose and lignin contents.Despite that SM83 mutant remained a similar biomass digestion rate,it contained an increased soluble sugar level by 14.99%,compared with wild type.Hence,the sweet sorghum mutants will be used for breeding energy sweet sorghum.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2015年第5期1-6,共6页
Journal of Huazhong Agricultural University
基金
教育部、国家外国专家局高等学校学科创新引智计划项目(B08032)
国家“973”计划前期研究专项(2010CB134401)
关键词
甜高粱
EMS突变体库
植物细胞壁
生物质降解
能源作物
sweet sorghum
EMS mutagenesis pool
plant cell wall
biomass digestibility
bioenergy crop
