摘要
目的：探讨非诺贝特对酿酒酵母基因表达谱的影响，在基因表达水平上分析其作用机理。方法：采用非诺贝特（100μg/mL）处理酿酒酵母90min后，抽提细胞RNA进行反转录荧光标记cRNA，与基因表达谱芯片进行杂交，用激光扫描仪检测杂交信号，并用相应软件进行分析。结果：与正常对照组相比，非诺贝特导致19个已知的基因表达明显上升（诱导倍数〉1.5），同时导致14个已知的基因表达明显下降（抑制倍数〉1.5）；另外，非诺贝特显著诱导了α-亚麻酸、脂肪酸代谢和降解信号通路，抑制了丙酮酸和碳代谢、糖酵解和糖异生、二羧酸代谢等信号通路（P〈0.05）。结论：非诺贝特可以明显调节脂肪酸、糖和尿酸代谢信号通路。
Objective：To investigate the effect of fenofibrate on saccharomyces cerevisiae gene expression profile and study the mechanism at gene expression level. Methods：After Saccharomyces cerevisiae was treated with fenofibrate （100μg/mL） for 90min, cell RNA was extracted and re-verse-transcripted into fluorescently-labeled cRNA which was hybridized with gene expression profile chip. Furthermore, laser scanner was used to detect the hybridization signal. Finally, data was analyzed by related software. Results：Compared with normal control group, fenofibrate obviously increased expression of 19 known genes （fold〉1.5）, and markedly reduced expression of 14 known genes （fold〉1.5）;in addition, fenofibrate signifi-cantly elevated the alpha-Linolenic acid metabolism, fatty acid degradation and metabolism signal pathway and decreased pyruvate metabolism, gly-colysis/gluconeogenesis and glyoxylate and dicarboxylate metabolism signal pathway （P〈0.05）. Conclusion：The results suggest that the fatty acid, glycogen and uric acid metabolism signal pathways could be significantly regulated by fenofibrate.
出处
《按摩与康复医学》
2015年第14期76-78,共3页
Chinese Manipulation and Rehabilitation Medicine
基金
广东省科技厅促进科技服务业发展计划项目,编号:2012B040304012
关键词
非诺贝特
酿酒酵母
基因表达谱
芯片
fenofibrate
saccharomyces cerevisiae
gene expression profile
chip
