摘要
目的探讨脑疝患者外周血CD8+CD28-T细胞的动态变化与临床意义。方法收集36例脑疝组(A组)患者外周血,以41例未出现脑疝的重型颅脑损伤患者(B组)及32例健康人(C组)作为对照,采用流式细胞术检测外周血CD8+CD28-T细胞及其胞内转化生长因子β1(TGF-β1)及白介素10(IL-10)百分含量。分别在伤后3 h、6 h及12 h观察A及B组患者外周血CD8+CD28-T细胞、TGF-β1及IL-10变化。结果三组CD8+CD28-T细胞、TGF-β1及IL-10总体差异均有显著性(P<0.05),但B组与C组无统计学差异,而A组上述三者均显著低于另外B及C组(P<0.05)。B组患者在伤后3 h、6 h及12 h的CD8+CD28-T细胞、TGF-β1及IL-10差异均无统计学意义;A组患者3 h、6 h及12 h的CD8+CD28-T细胞、TGF-β1及IL-10均呈进行性下降,表现为任意两个时间点的差异均有统计学意义(P<0.05),且均显著低于B组(P<0.05)。结论 CD8+CD28-T细胞显著下降是脑疝患者的一个重要细胞免疫学特征,其伤后12h内进行性下降与病情进展密切相关。
Objective To investigate the dynamic changes of peripheral blood CD8^＋CD28^-T cells and clinical significance in patients with cerebral hernia. Methods A total of 77 patients of severe brain injury were divided into two groups ： cerebral hernia（A group, n =36） and no-cerebral hernia（B group, n =41）, 32 healthy subjects were in control group（C group）, the level of CD4＋CD25＋and the percentage of TGF-β1, IL-10 in peripheral blood was analyzed with flow cytometry.All variables were observed in group A and B at post-traumatic 3, 6, 12 h. Results Difference of three groups in CD8^＋CD28^-T cells, TGF-β1 and IL-10 overall was significant（P 0.05）, but no statistically significant difference between group B and group C, all variables in group A was significantly lower than group B and group C（P〈0.05）. The level of CD8^＋CD28^-T cells, TGF-β1 and IL-10 had no significant difference in group B for 3, 6, 12 h. Compared with group B, CD8^＋CD28^-T cells, TGF-β1 and IL-10 in group A of three different time points showed a progressive decline, difference between any two time points was significant. Conclusion One important cellular immunological characteristics of cerebral hernia is the significant decrease of CD8^＋CD28^-T cells, especially the progressive decline of which within 12 h are closely related to disease progression.
出处
《岭南现代临床外科》
2015年第4期507-510,共4页
Lingnan Modern Clinics in Surgery
基金
广东省产业技术研究与开发资金计划项目(编号:2011B031800048)
