摘要
近年来,作为非标记的荧光探针,以DNA为模板制备的荧光纳米材料因其具有光稳定性、生物相容性、无毒性等优点,被广泛应用于生物分析领域。针对一些检测生物酶存在的成本高、灵敏度低、操作复杂等问题,本文基于焦磷酸(PPi)对聚T链为模板的荧光铜纳米颗粒的形成具有很强的抑制作用,发展了一种非标记的荧光增强的方法用来检测碱性磷酸酯酶活性。该方法不仅直接在均相溶液中就可进行,而且所需时间短,成本低廉。其检测下限达到0.5U·L-1。
Recently, a type of newly emerged functional fluorescent probe, DNA-templated metal nanomaterials, had widely been used for label-free detection of biomarkers due to their outstanding optical properties, low toxicity, facile surface modification and good biocompatibility. In order to solve some problems existed in the assay of biological enzyme, such as high cost, low sensitivity, and complex operation, a novel label-free fluorescent method for MTase activity assay by coupling double-stranded DNA（ds DNA）-templated copper nanoparticles（Cu NPs） with endonuclease-assisted signal transduction system had been developed. This strategy was also demonstrated to provide excellent platform to screen the inhibitors for Dam MTase.
出处
《化工技术与开发》
CAS
2015年第7期10-13,47,共5页
Technology & Development of Chemical Industry
关键词
碱性磷酸酯酶
铜纳米颗粒
alkaline phosphatase
copper nanoparticles
