摘要
目的对玫瑰Rosa rugosa花蕾中的黄酮类化合物进行研究。方法运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术对食用玫瑰花蕾的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从玫瑰花蕾95%乙醇提取物的正丁醇萃取部位分离得到了9个黄酮类化合物,分别鉴定为8-乙酰基-4′,7-二甲氧基-6-甲基黄酮(1)、山柰酚(2)、山柰酚-3-O-β-D-吡喃葡萄糖苷(3)、山柰酚-3-O-β-D-芸香糖苷(4)、槲皮素(5)、槲皮素-3-O-β-D-吡喃葡萄糖苷(6)、芦丁(7)、木犀草素(8)、木犀草素-7-O-β-D-吡喃葡萄糖苷(9)。结论化合物1为新化合物,化合物9为首次从玫瑰花蕾中分离得到,并且化合物1表现出较强的细胞毒活性,其对NB4、SH-SY5Y、PC3、A549和MCF7的IC50值分别为6.8、5.6、2.2、1.8、7.4μmol/L。
Objective To identificate the bioactive natural products, and study the chemical constituents in the flower buds of Rosa rugosa. Methods The chemical constituents in the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography （HPLC） methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results Nine flavonoids were isolated from the flower buds ofR. rugosa, and identified as 8-acetyl-4＇,7-dimethoxy-6-methyl-flavone （1）, kaempferol （2）, kaempferol-3-O-β-D-glucopyranoside （3）, kaempferol-3-O-β-D-rutinside （4）, quercetin （5）, quercetin-3-O-β-D-glucopyranoside （6）, rutin （7）, luteolin （8）, and luteolin-7-O-β-D-glucopyranoside （9）. Conclusion Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SYSY, PC3, A549, and MCF7 cells with ICso values of 6.8, 5.6, 2.2, 1.8, and 7.4 μmol/L, respectively. Compound 9 is isolated from R. rugosa for the first time.
出处
《中草药》
CAS
CSCD
北大核心
2015年第13期1867-1871,共5页
Chinese Traditional and Herbal Drugs
基金
云南民族大学研究生创新项目基金(2014YJY73)
