摘要
目的探讨青蒿琥酯(Ast)对实验性自身免疫性脑脊髓炎(EAE)大鼠脑组织前列腺素E2(PGE2)表达的影响。方法选取Wistar雌性大鼠50只,随机分为正常对照组、EAE对照组、Ast大剂量组、Ast中剂量组、Ast小剂量组,各10只。EAE对照组、Ast大剂量组、Ast中剂量组、Ast小剂量组进行EAE造模。正常对照组和EAE对照组灌服0.9%氯化钠注射液,Ast大剂量组、Ast中剂量组、Ast小剂量组分别予以20mg·kg-1·d-1、10mg·kg-1·d-1、5mg·kg-1·d-1的Ast。观察各组大鼠发病潜伏期、进展期及神经功能障碍评分,检测脑组织PGE2表达。结果 5组大鼠发病潜伏期、进展期及神经功能障碍评分比较,差异有统计学意义(P<0.01);Ast大剂量组、Ast中剂量组、Ast小剂量组大鼠发病潜伏期长于EAE对照组,进展期短于EAE对照组,神经功能障碍评分低于EAE对照组;Ast大剂量组、Ast中剂量组大鼠发病潜伏期长于Ast小剂量组,进展期短于Ast小剂量组,神经功能障碍评分低于Ast小剂量组;Ast大剂量组大鼠发病潜伏期长于Ast中剂量组,进展期短于Ast中剂量组,神经功能障碍评分低于Ast中剂量组,差异均有统计学意义(P<0.05)。正常对照组大鼠脑组织未见PGE2表达,EAE对照组、Ast大剂量组、Ast中剂量组、Ast小剂量组可见PGE2表达。5组大鼠脑组织PGE2表达的平均光密度值比较,差异有统计学意义(P<0.05);Ast大剂量组、Ast中剂量组、Ast小剂量组大鼠脑组织PGE2表达的平均光密度值小于EAE对照组,Ast大剂量组、Ast中剂量组大鼠脑组织PGE2表达的平均光密度值小于Ast小剂量组,Ast大剂量组大鼠脑组织PGE2表达的平均光密度值小于Ast中剂量组,差异均有统计学意义(P<0.05)。PGE2表达与大鼠发病潜伏期呈负相关(r=-0.867,P<0.05);PGE2表达与大鼠发病进展期呈正相关(r=0.913,P<0.05);PGE2表达与大鼠神经功能障碍评分呈正相关(r=0.893,P<0.05)。结论 Ast能改善EAE大鼠临床症状,防止EAE�
Objective To explore the impact of artesunate（Ast）on the expression of prostaglandin E2 （PGE2 ）in the brain tissue of Wistar rats with experimental autoimmune encephalomyelitis（EAE）. Methods A total of 50 female wistar rats were randomly divided into normal control group,EAE control group,low dose Ast group,median dose Ast group and high dose Ast group,10 rats in each group. EAE control group,low dose Ast group,median dose Ast group and high dose Ast group were made as EAE model. Normal control group and EAE control group were given 0. 9% sodium chloride injection,low dose Ast group,median dose Ast group and high dose Ast group were respectively given Ast of 20mg·kg^- 1·d^-,10mg·kg^- 1 ·d^- 1 ,5mg·kg ^- 1·d ^- 1 . The latent period,progressive stage and neurological deficits scores of each group were compared, brain tissue PGE2 expression was detected. Results The latent period,progressive stage and neurological deficits score of the five groups were compared,differences were statistical significance（P ﹤ 0. 01）;the latent period of low dose Ast group,medi-an dose Ast group and high dose Ast group were longer than those of EAE control group,progressive stage were shorter than those of EAE control group,neurological deficits score were lower than that of EAE control group;the latent period of median dose Ast group and high dose Ast group were longer than those of low does Ast group,progressive stage were shorter than those of low dose Ast group,neurological deficits score was lower than that of low dose Ast group;the latent of period high dose Ast group was longer than that of low median Ast group,progressive stage was shorter than that of median dose Ast group,neurological deficits score was lower than that of median dose Ast group（P ﹤ 0. 05）. There was no expression of PGE2 in the normal control group, there were expression of PGE2 in the EAE control group,low dose Ast group,median dose Ast group and high dose Ast group.
出处
《临床合理用药杂志》
2015年第18期18-20,共3页
Chinese Journal of Clinical Rational Drug Use
