摘要
目的观察慢性乙型肝炎患者(CHB)CISH、SOCS1和SOCS3 m RNA及CD4+T淋巴细胞亚群相关细胞因子的表达,探讨CISH、SOCS1和SOCS3在慢性乙型肝炎病毒(HBV)感染中的作用。方法选取慢性乙型肝炎患者(CHB)31例,乙肝携带者(As C)18例,正常健康者18例作为对照,采用实时荧光定量PCR的方法检测外周血单个核细胞(PBMC)中CISH、SOCS1和SOCS3 m RNA的表达,ELISA法检测PBMC上清中IL-2、IL-4、IFN-γ、IL-10、IL-17A和TGF-β细胞因子的表达水平,并分析其相关性。结果CHB组患者CISH m RNA表达高于健康对照组(P<0.01),CHB组SOCS3 m RNA表达显著高于健康对照组(P<0.01),较As C组高(P<0.05),SOCS1 m RNA表达量在各组间比较无明显差异(P>0.05);CHB组IL-4的表达量高于As C组,CHB组IFN-γ/IL-4的比值低于健康对照组,As C组IL-4和TGF-β的表达量均高于健康对照组,差异均有统计学意义(P<0.05),CHB组IL-4、IL-17A和TGF-β的表达量均显著高于健康对照组,差异有显著统计学意义(P<0.01);CHB组患者SOCS1 m RNA表达量与CISH m RNA表达量和SOCS3 m RNA表达量之间均存在显著性关联(P=0.004,P=0.002),SOCS3 m RNA表达量与IFN-γ水平之间存在相关性(P=0.037)。结论 CISH和SOCS3基因可能参与慢性乙型肝炎疾病的发生发展,并与CD4+T淋巴细胞亚群变化之间存在一定的相关性。
This study designed to investigate the expressions of CISH, SOCS1, SOCS3 m RNA and CD4+T lymphocyte subsets related cytokines in patients with chronic hepatitis B virus infection, and to evaluate the possible roles of these genes in HBV infection. Blood samples of 31 chronic hepatitis B(CHB) patients, 18 chronic asymptomatic HBV carriers(As C), and 18 healthy subjects(normal control) were collected, the transcript levels of CISH, SOCS1, SOCS3 in peripheral blood mononuclear cells(PBMCs) were determined by SYBR Green quantitative RT-PCR, and the expressions of IL-2, IL-4, IFN- γ, IL-10, IL-17 A and TGF-βin supernatant of PBMCs were measured by ELISA technique. Data showed that the expression of CISH/SOCS3 m RNA in of CHB group was significantly higher than that in control group(P〈0.01), and the expression of SOCS3 m RNA in CHB group was higher than that in As C group(P〈0.05), while the expression of SOCS1 m RNA showed no statistically different between the three groups(P〈0.05). The level of IL-4 in CHB group was higher than that in As C group(P〈0.05). While compared with control group, CHB group showed a lower level of IFN-γ/IL-4 ratio(P〈0.05), higher levels IL-4, IL-17 A and TGF-β( P〈0. 01), and As C group showed a higher levels of IL-4 and TGF-β(P〈0.05). In addition, the transcript level of SOCS1 m RNA was correlated with CISH and SOCS3, and there was weakly positive correlation between SOCS3 and IFN-γ(P =0.004, P =0.002, P =0.037, respectively). In conclusion, CISH and SOCS3 genes may be involved in the development of chronic hepatitis B infection, and have certain correlation with the changes of CD4+T lymphocyte subsets related cytokines.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2015年第8期677-681,共5页
Immunological Journal
基金
成都医学院第一附属医院独立资助项目(CYFY14DL-007)
四川省教育厅资助项目(13ZA0211)
关键词
乙型肝炎
CISH
SOCS1
SOCS3
TH1/TH2
Hepatitis B virus
Cytokine-inducible SH2 protein
Suppressors of cytokine signaling 1
Suppressors of cytokine signaling 3
Th1/Th2