期刊文献+

慢性乙型肝炎患者CISH、SOCS1和SOCS3 mRNA及CD4^+T淋巴细胞亚群相关细胞因子的表达 被引量:8

The expression of cytokine signaling suppressors m RNA and CD4^+T lymphocyte subsets related cytokines in patients with chronic hepatitis B infection
下载PDF
导出
摘要 目的观察慢性乙型肝炎患者(CHB)CISH、SOCS1和SOCS3 m RNA及CD4+T淋巴细胞亚群相关细胞因子的表达,探讨CISH、SOCS1和SOCS3在慢性乙型肝炎病毒(HBV)感染中的作用。方法选取慢性乙型肝炎患者(CHB)31例,乙肝携带者(As C)18例,正常健康者18例作为对照,采用实时荧光定量PCR的方法检测外周血单个核细胞(PBMC)中CISH、SOCS1和SOCS3 m RNA的表达,ELISA法检测PBMC上清中IL-2、IL-4、IFN-γ、IL-10、IL-17A和TGF-β细胞因子的表达水平,并分析其相关性。结果CHB组患者CISH m RNA表达高于健康对照组(P<0.01),CHB组SOCS3 m RNA表达显著高于健康对照组(P<0.01),较As C组高(P<0.05),SOCS1 m RNA表达量在各组间比较无明显差异(P>0.05);CHB组IL-4的表达量高于As C组,CHB组IFN-γ/IL-4的比值低于健康对照组,As C组IL-4和TGF-β的表达量均高于健康对照组,差异均有统计学意义(P<0.05),CHB组IL-4、IL-17A和TGF-β的表达量均显著高于健康对照组,差异有显著统计学意义(P<0.01);CHB组患者SOCS1 m RNA表达量与CISH m RNA表达量和SOCS3 m RNA表达量之间均存在显著性关联(P=0.004,P=0.002),SOCS3 m RNA表达量与IFN-γ水平之间存在相关性(P=0.037)。结论 CISH和SOCS3基因可能参与慢性乙型肝炎疾病的发生发展,并与CD4+T淋巴细胞亚群变化之间存在一定的相关性。 This study designed to investigate the expressions of CISH, SOCS1, SOCS3 m RNA and CD4+T lymphocyte subsets related cytokines in patients with chronic hepatitis B virus infection, and to evaluate the possible roles of these genes in HBV infection. Blood samples of 31 chronic hepatitis B(CHB) patients, 18 chronic asymptomatic HBV carriers(As C), and 18 healthy subjects(normal control) were collected, the transcript levels of CISH, SOCS1, SOCS3 in peripheral blood mononuclear cells(PBMCs) were determined by SYBR Green quantitative RT-PCR, and the expressions of IL-2, IL-4, IFN- γ, IL-10, IL-17 A and TGF-βin supernatant of PBMCs were measured by ELISA technique. Data showed that the expression of CISH/SOCS3 m RNA in of CHB group was significantly higher than that in control group(P〈0.01), and the expression of SOCS3 m RNA in CHB group was higher than that in As C group(P〈0.05), while the expression of SOCS1 m RNA showed no statistically different between the three groups(P〈0.05). The level of IL-4 in CHB group was higher than that in As C group(P〈0.05). While compared with control group, CHB group showed a lower level of IFN-γ/IL-4 ratio(P〈0.05), higher levels IL-4, IL-17 A and TGF-β( P〈0. 01), and As C group showed a higher levels of IL-4 and TGF-β(P〈0.05). In addition, the transcript level of SOCS1 m RNA was correlated with CISH and SOCS3, and there was weakly positive correlation between SOCS3 and IFN-γ(P =0.004, P =0.002, P =0.037, respectively). In conclusion, CISH and SOCS3 genes may be involved in the development of chronic hepatitis B infection, and have certain correlation with the changes of CD4+T lymphocyte subsets related cytokines.
出处 《免疫学杂志》 CAS CSCD 北大核心 2015年第8期677-681,共5页 Immunological Journal
基金 成都医学院第一附属医院独立资助项目(CYFY14DL-007) 四川省教育厅资助项目(13ZA0211)
关键词 乙型肝炎 CISH SOCS1 SOCS3 TH1/TH2 Hepatitis B virus Cytokine-inducible SH2 protein Suppressors of cytokine signaling 1 Suppressors of cytokine signaling 3 Th1/Th2
  • 相关文献

参考文献18

  • 1Wright TL. Introduction to chronic hepatitis B infection[J]. Am J Gastroenterol, 2006, 101: S1. 被引量:1
  • 2Shimada K, Serada S, Fujimoto M, et al. Molecular mechanism underlying the antiproliferative effect of suppressor of cytokine signaling-1 in non-small-cell lung cancer cells[J]. Cancer Sci, 2013, 104(11): 1483-1491. 被引量:1
  • 3Culiq Z. Suppressors of cytokine signalling-3 and -1 in human carcinogenesis[J]. Front Biosci (Schol Ed), 2013, 5: 277-283. 被引量:1
  • 4Gu H, Liu L, Ma S, et al. Inhibition of SOCS-3 in adipoeytes of rats with diet-induced obesity increases leptin-mediated fatty acid oxidation[J]. Endocrine, 2009, 36(3): 546-554. 被引量:1
  • 5Jorgensen SB, O'Neill HM, Sylow L, et al. Deletion of skeletal muscle SOCS3 prevents insulin resistance in obesity[J]. Diabetes, 2013, 62(1): 56-64. 被引量:1
  • 6Kuzmicki M, Telejko B, Wawrusiewiez Kurylonek N, et al. The expression of suppressor of eytokine signaling 1 and 3 in fat and placental tissue from women with gestational diabetes[J]. Gynecol Endocrinol, 2012, 28(11): 841-844. 被引量:1
  • 7Suehy D, Labuzek K, Maehnik G, et al. SOCS and diabetes-ups and downs of a turbulent relationship[J]. Cell Biochem Funet, 2013, 31(3): 181-195. 被引量:1
  • 8Harada M, Nakashima K, Hirota T, et al. Functional polymorphism in the suppressor of cytokine signaling 1 gene associated with adult asthma[J]. Am J Respir Cell Mol Biol, 2007, 36(4): 491-496. 被引量:1
  • 9Zhang X, Wang J, Cheng J, et al. An integrated analysis of SOCS1 down -regulation in HBV infection -related hepatoeellular carcinoma[J]. J Viral Hepat, 2014, 21(4): 264-271. 被引量:1
  • 10Koeberlein B, zur Hausen A, Bektas N, et al. Hepatitis B virus overexpresses suppressor of cytokine signaling-3 (SOCS3) thereby contributing to severity of inflammation in the liver[J]. Virus Res, 2010, 148(1): 51-59. 被引量:1

同被引文献80

引证文献8

二级引证文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部