摘要
以脱壳大麦为原料,采用Obsorne法提取大麦醇溶蛋白,探讨了乙醇体积分数、液料比、提取温度、提取时间对大麦醇溶蛋白提取率和纯度的影响。实验结果表明:在乙醇的体积分数70%、液料比(m L/g)6:1、温度25℃、提取时间2.0 h条件下,大麦醇溶蛋白纯度为82.1%,提取率为2.95%。大麦醇溶蛋白的理化性质检测结果表明,大麦醇溶蛋白具有良好的持油性、乳化性及乳化稳定性,但是疏水性较弱。
Osborn method was used to isolate hordein from hulled barley. Discusses the ethanol concentration, liquid ratio, temperature and extraction time on the hordein purity and extraction yield. The optimum conditions for hordein extraction were found to be extraction at 25 ℃ for 2.0 h using 70% ethanol volume fraction at a solvent-solid ratio of 6：1 （mL/g）, under the condition hordein purity is 82.1%, yield is 2.95%. The results of hordein properties show that hordein have a good capacity holding oil, emulsification and emulsion stability, but weaker hydrophobic.
出处
《粮食加工》
2015年第4期40-43,共4页
Grain Processing
关键词
大麦醇溶蛋白
提取
乳化性
氨基酸组成
hordein
extraction
emulsification
amino acid composition
