摘要
利用Micro SAtellite软件分析筛选芒果转录组中的SSR位点。结果表明,在54 207条Unigene中共搜索得到4 103个SSR位点,出现频率为7.57%。其中,三核苷酸重复为主导重复类型,占SSR总数的38.19%,其次是单核苷酸重复(26.91%)。AG/CT和AAG/CTT分别是二核苷酸和三核苷酸重复的优势基元。根据SSR侧翼序列共设计6 915对SSR引物,随机挑选了230对进行PCR检测,获得93对多态性引物。由此可见,芒果转录组数据可以作为大量开发SSR标记的资源,这些SSR标记也将有助于芒果遗传多样性和种质资源鉴定的研究。
A MicroSAtellite software was used to find the SSR loci in the mango transcriptome. As a result, a total of 4 103 SSRs were found from 54 207 unigenes, with a frequency of 7.57%. The trinucleotide repeats were the most frequent SSRs among them （38.19%）. AG/CT and AAG/CTF were the most common motifs in the dinucleotide and trinucleotide repeats, respectively. Based on the flanking sequences of these SSR loci, 6 915 SSR primer pairs were designed. A total of 230 primer pairs were randomly selected to be verified by PCR, and 93 pairs （40.43%） of them exhibited to be polymorphism. The results showed that transcriptome data would be an effective source for the exploration of SSR markers for mango and these SSR markers would contribute greatly to the research into genetic diversity and germplasm characterization in mango.
出处
《热带作物学报》
CSCD
北大核心
2015年第7期1261-1266,共6页
Chinese Journal of Tropical Crops
基金
农业行业科研专项(No.201203092-3)
中央级公益性科研院所基本科研业务费(No.1630062014008
No.1630062013003)
"广东省自然科学基金-博士启动(No.2014A030310341)"
"海南省自然科学基金(No.20153044)"
关键词
芒果
转录组
SSR信息
多态性
Mango
Transcriptome
SSR information
Polymorphism
