摘要
目的采用JPK Nano Wizard 3型生物原子力显微镜(AFM)对两种类型的山羊颞下颌关节盘细胞(软骨细胞样细胞和成纤维细胞样细胞)的表面形貌和生物力学特性进行研究,为颞下颌关节盘组织工程研究中种子细胞的选择提供依据。方法单层培养原代山羊颞下颌关节盘细胞,生理状态下用AFM接触模式扫描两种类型的关节盘细胞,获得形貌图。随机选取20个软骨细胞样细胞和成纤维细胞样细胞,扫描获得细胞核区和细胞质区的力-位移曲线,通过JPK Data Processing软件分析两种细胞的杨氏模量和黏附力。结果 AFM下,三角形的软骨细胞样细胞的细胞核占据其大部分体积,细胞骨架结构呈树枝状排列,边缘伸出许多伪足;长梭形的成纤维细胞样细胞的细胞核明显高于细胞质区,骨架结构规则排列,边缘光滑,极少有伪足伸出;两种细胞的表面粗糙度没有明显差异。分析力-位移曲线得出:两种类型细胞的杨氏模量在细胞核区没有明显差异(P>0.05),在细胞质区略有差异(P=0.047);但两种细胞的黏附力没有明显差异(P>0.05)。结论两种类型的关节盘细胞在形貌上虽有较大的差别,但在生物力学特性上的差异并不明显。
Objective This study is performed to investigate the cell topographies and biomechanical properties of two different types of temporomandibular joint（TMJ） discs from goats by using JPK Nano Wizard 3 biological atomic force microscopy（AFM）. This process provides a guideline for selecting seed cells for TMJ disc tissue engineering. Methods TMJ disc cells from primary goats were cultured by monolayer culture method. AFM was used to contact scan the topographies of the two types of TMJ disc cells under physiological environment. Approximately 20 chondrocyte-like and fibroblast-like cells were selected randomly to plot the force-versus-distance curves of the cytoplasm and nucleus. Young＇s modulus and adhesion were analyzed by JPK Data Processing. Results The triangle-shapednucleus of the chondrocyte-like cell occupied a large portion of the cell. Cytoskeleton was arranged dendritically on the surface. Pseudopodia were extended from cell edges. The spindle-shapednucleus of the fibroblast-like cell occupied a significantly larger region compared with the cytoplasmic region. Cytoskeleton was arranged regularly. Cell edges were smooth with less pseudopodia extended. No difference was found in the surface roughness between the two types of cells. According to the force-versus-distance curves, the Young＇s moduli of the two types of cells were not statistically different（P0.05）, but differences were found in the cytoplasmic regions（P=0.047）. No statistical difference was found in the adhesions between the two types of cells（P0.05）. Conclusion The AFM topography and curves were compared and analyzed. The two types of TMJ disc cells exhibited significantly different topographies, but only slight difference in their mechanical abilities.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2015年第4期352-356,共5页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(81160139)
关键词
原子力显微镜
关节盘细胞
表面形貌
生物力学
atomic force microscopy
temporomandibular joint disc cell
topography
biomechanics
