摘要
采用沙培的方法,以最适锰浓度为对照,设置重度锰缺乏(对照浓度的1/25,S1表示)、中度锰缺乏(对照浓度的1/5,S2表示)、中度锰过量(对照浓度的5倍,S3表示)、重度锰过量(对照浓度的25倍,S4表示)不同程度锰缺乏和锰过量处理,研究了锰胁迫对大豆氮代谢关键酶活性的影响。结果表明:不同程度锰缺乏胁迫抑制了叶片GS和GOGAT活性,而锰过量胁迫则起促进作用;锰缺乏胁迫对根GS无显著影响,锰过量胁迫则起抑制作用;不同程度锰胁迫对根GOGAT活性均起抑制作用,而对茎GS和GOGAT活性则无显著影响;重度锰胁迫(S1和S4)对叶片GDH起促进作用,轻度锰胁迫(S2和S3)起抑制作用;不同程度锰胁迫对茎和根GDH活性均起抑制作用。综合分析表明,大豆叶、茎、根GS、GOGAT、GDH活性对锰胁迫的响应不同,这可能与上述氮代谢相关酶在不同器官中的氮代谢功能、同工酶组成不同有关,并最终导致大豆氮代谢状况发生改变。
Soybean was cultivated with sand culture method,and optimal Mn concentration was used as control,and 4 manganese stress treatments including 2 manganese deficiency treatments（ including serious stress and light stress which were 1 /25 and 1 /5 of optimal manganese concentration respectively,expressed by S1 and S2） and 2 excess stress treatments（ including serious stress and light stress which were 5 and 25 times of optimal Mn concentration respectively,expressed by S3 and S4）were set to study the effect of manganese stress on activities of enzymes involved in nitrogen metabolism. The results showed that： activities of GS and GOGAT in leaf were inhibited by manganese deficiency stress in different degree,but promoted by manganese excess stress. Activities of GS of root was not affected by manganese deficiency stress（ S1 and S2）,but inhibited by manganese excess stress（ S3 and S4）. All Manganese stress significantly inhibited the activities of GOGAT in root,but had no effect on the activities of GS and GOGAT in stem. Activities of GDH in leaf were promoted by serious manganese stress（ S1 and S4）,while inhibited by light manganese stress（ S2 and S3）. All manganese stress inhibited the activities of GDH in stem and root. In conclusion,the activities of GS,GOGAT,GDH in leaves,stems,roots were all influenced by manganese stress in different form because of different metabolic function and composition of isoenzymes in different organs,which lead the change of nitrogen metabolic state.
出处
《大豆科学》
CAS
CSCD
北大核心
2015年第3期432-435,共4页
Soybean Science
基金
国家自然科学基金(204134008)
黑龙江八一农垦大学博士启动基金(XYB2013-03)
黑龙江八一农垦大学省作物学重点学科学术骨干科研启动金
