摘要
【目的】比较酿酒葡萄品种的抗寒性强弱,筛选出具有较好抗寒性的品种。【方法】以引进的3个酿酒葡萄品种‘北冰红’、‘左优红’、‘双红’以及石河子主栽品种‘赤霞珠’的1 a(年)生枝条为材料,测定低温处理后各品种的相对电导率、可溶性糖、可溶性蛋白、脯氨酸、丙二醛、超氧化物歧化酶、过氧化物酶及过氧化氢酶的活性,应用电导法配合Logistic方程计算供试品种的半致死温度,并采用方差分析、主成分分析对不同品种的抗寒力进行比较。【结果】4个品种的相对电导率和丙二醛含量随着温度的降低而升高,SOD活性、可溶性糖含量和脯氨酸含量先升后降,CAT活性和可溶性蛋白含量一直升高,POD活性下降。【结论】通过主成分分析综合评判4个品种抗寒性大小依次为‘双红’>‘左优红’>‘北冰红’>‘赤霞珠’,研究结果为酿酒葡萄的引种与栽培管理提供了参考依据。
【Objective】To study the differences of cold resistance among grape cultivars and select wine va-rieties with good cold resistance.【Methods】The shoots of three introduced wine grape cultivars‘Beibing-hong＇,‘Zuoyouhong＇,‘Double Red＇and local major cultivar‘Cabernet Sauvignon＇were used as exper-iment materials. The relative conductivity,soluble sugar,soluble protein,proline,malondialdehyde,su-peroxide dismutase,peroxidase and catalase activity of different cultivars were measured after low temper-ature treatment. The semi lethal temperatures of the tested cultivars were calculated by the conductancemethod with application of Logistic equation,and the cold resistance of different cultivars was comparedwith variance and principal component analysis.【Results】The results showed that the relative conductivi-ty and MDA content of 4 cultivars increased with the decrease of temperature,and the activity of SOD,content of soluble sugar and proline increased first and then decreased. CAT activity and soluble proteincontent kept increase,while POD activity decreased.【Conclusion】Through principal component analy-sis,the order of cold resistance of 4 cultivars was‘Double Red＇‘Zuoyouhong＇‘Beibinghong＇‘Cabernet Sauvignon＇,which could provide reference for introduction and cultivation management ofwine grape.
出处
《果树学报》
CAS
CSCD
北大核心
2015年第4期612-619,共8页
Journal of Fruit Science
基金
石河子大学重大科技攻关计划项目(gxjs2012-zdgg04-05)
关键词
酿酒葡萄
抗寒性
其他主成分分析
半致死温度
Wine grapevine
Cold resistance
Principal component analysis
Semilethal temperature
