摘要
目的建立毛细管气相色谱法测定还原型谷胱甘肽中4种有机溶剂的残留量。方法采用气相色谱法,DB-624毛细管柱(60m×0.25mm×0.25μm),FID检测器,柱温为程序升温,以DMF-水(3∶7)为溶剂,测定了还原型谷胱甘肽原料药中甲醇、乙醇、乙酸乙酯、四氢呋喃4种有机溶剂的残留量。结果 4种有机溶剂均能完全分离,在所考察的范围内线性关系良好,加样回收率均满意。结论该方法灵敏、准确,适用于还原型谷胱甘肽原料中有机溶剂残留量的检测。
Objective To establish a gas chromatography（GC）method for the determination of residual organic solvents in reduced glutathione.Method Capillary gas chromatography（GC）was applied.The residues of menthanol,ethanol,dichlormethane and tetrahydrofuran were determined by GC with DB-624 capillary column（60m×0.25mm×0.25μm）,and hydrogen flame ionization detector（FID）.The column temperature was programmed.The solvent was DMF-water（3∶7）.Results The four solvents were separated completely and showed a linear relationship in the investigated range.Satisfactory recovery rate were obtained.Conclusion The established method is sensitive,accurate and suitable for the determination of residual organic solvents in reduced glutathione.
出处
《西北药学杂志》
CAS
2015年第4期369-371,共3页
Northwest Pharmaceutical Journal
