摘要
目的研究不同自主神经干预对心房神经节活性的影响。方法 24只健康成年家犬随机分为四组：低强度耳屏刺激（LL-TS）组（n=6）、低强度颈动脉窦压力感受器刺激（LL-CBS）组（n=6）、低强度脊髓神经刺激（LL-SCS）组（n=6）和对照组（n=6）。各组分别给予自主神经干预：LL-TS组通过连接于刺激仪的自制耳夹以降低窦性心率阈电压80%对双侧耳屏给予耳屏刺激3 h;LL-CBS对颈动脉窦压力感受器以使血压下降的阈电压80%进行LL-CBS 3 h;LL-SCS组在T1-5脊髓节段放置刺激电极以使肌肉收缩的阈电压80%给予SCS 3 h;对照组不给予干预观察3 h。别在基础状态和3 h干预后,将电极置于左上神经节（SLGP）发放高频电刺激（HFS）诱导窦性心率减慢,将微电极插入右前神经节（ARGP）记录神经放电,分测定各组SLGP的功能和ARGP的活性。结果 LL-TS、LL-CBS和LL-SCS降低了高频刺激SLGP产生的减慢窦率作用,并且抑制了从ARGP记录到的神经活性频率和振幅。结论 LL-TS、LL-CBS和LL-SCS自主神经干预方法可以抑制心房神经节的功能和活性。
Objective To investigate the effects of different autonomic nervous intervention on the activity of atrial ganglionated plexi（GP）. Methods Twenty-four dogs were randomly divided into four groups： the low-level tragus stimulation（LL-TS） group（n=6）, the low-level carotid baroreceptor stimulation（LL-CBS） group（n=6）, the low-level spinal cord stimulation group（n=6） and the control group（n=6）. LL-TS was delivered to bilateral tragus with ear-clips connected to a custom-made stimulator at voltages 80% below the threshold which could slow sinus rate for 3 hours. LL-CBS was delivered to the carotid baroreceptor at voltages 80% below the threshold which could reduce BP for 3 hours. LL-SCS was delivered to the T1-5via wire electrodes at voltages 80% below the threshold which could cause muscular twitching for 3 hours. No intervention was performed to the dogs in control group. One electrode was attached to the superior left GP（SLGP） to deliver high frequency stimulation（HFS）, and the maximum heart rate slowing change was measured as the function of SLGP. Microelectrodes were inserted into the anterior right GP（ARGP） to record neural firing. Results LL-TS, LL-CBS and LL-SCS decreased the HR slowing change when HFS was delivered to the SLGP. The neural activity of ARGP was also suppressed. Conclusion LL-TS, LL-CBS and LL-SCS could suppress the function and activity of atrial ganglionated plexi.
出处
《海南医学》
CAS
2015年第13期1873-1876,共4页
Hainan Medical Journal
基金
国家自然科学基金(编号:81270339
81300182)
武汉市科技攻关计划项目基金(编号:201306060201010271)
湖北省自然科学基金(编号:2013CFB302)
中央高校基本科研业务费专项资金(编号:2042012kf1099
2042014kf0110)
武汉大学博士研究生自主科研项目(编号:2012302020206)
关键词
自主神经系统
心房神经节
神经活性记录
脊髓神经刺激
Autonomic nervous system
Atrial ganglionated plexi
Neural activity recording
Spinal cord stimulation
