摘要
目的探讨Notch信号通路的两种配体DIL-4和Jagged-1与慢性丙型肝炎的关系。方法采集慢性丙型肝炎患者及健康对照组的新鲜空腹抗凝血,分离单个核细胞,提取RNA,反转录为c DNA,应用实时定量RT-PCR技术检测DIL-4和Jagged-1。结果慢性丙型肝炎患者组和正常对照组相比较,DIL-4和Jagged-1测定水平均明显升高,具有显著性差异,P<0.05。结论 Notch配体在慢性丙型肝炎中明显升高,且转氨酶越高,Notch配体检测水平越高,Notch配体与慢性丙型肝炎存在相关性。
Objective To explore the relationship of two kinds of notch signaling pathway ligand DIL-4 and Jagged-1 with chronic hepatitis C. Methods The fasting of anticoagulant fresh blood of patients with chronic hepatitis C and healthy control were collected. The separation of mononuclear cells, extraction of RNA and reverse transcription for eDNA were carried out and DIL-4 and Jagged-1 were determined by real-time quantitative RT-PCR. Results The DIL-4 and Jagged-1 levels in patients with chronic hepatitis C were significantly elevated than that of normal control（ P 〈 0.05 ）. Conclusion The Notch ligands in chronic hepatitis C increase significantly and the higher the transaminase, the higher the Notch li^ands levels.
出处
《标记免疫分析与临床》
CAS
2015年第7期657-659,共3页
Labeled Immunoassays and Clinical Medicine
基金
衡水市科技局"Notch信号通路在慢性丙型肝炎中检测的应用研究"(项目编号:13020z)
