摘要
目的观察川芎含药血清对血小板源生长因子(PDGF-BB)活化的大鼠肝星状细胞(HSCs)大麻素受体1(CB1)、黏着斑激酶(FAK)及磷酸化丝氨酸/苏氨酸蛋白激酶(p-Akt)蛋白表达的影响,探讨其抗纤维化的作用机制。方法制备川芎含药血清,MTT法检测川芎含药血清对肝星状细胞增殖情况,Western blot法分析肝星状细胞CB1、FAK、p-Akt蛋白的表达。结果与正常对照组相比,PDGF-BB作用24 h能刺激HSCs增殖,上调CB1、FAK、p-Akt蛋白的表达;川芎含药血清、秋水仙碱及大麻素受体1抑制剂AM251均能明显抑制上述效应;且加入AM251后,川芎含药血清对上述效应的抑制效果增强,且作用呈剂量依赖性。结论川芎含药血清可能通过大麻素相关信号通路抑制HSCs增殖,从而发挥防治肝纤维化的作用。
AIM To investigate the effects of serum containing Ligusticum chuanxiong on cannabinoid receptor1（ CB1）,focal adhesion kinase（ FAK） and extracellular regulated protein kinase（ p-Akt） in rat hepatic stellate cells（ HSCs） stimulated by platelet-derived growth factor（ PDGF-BB） and to explore the mechanism of hepatic fibrosis. METHODS The effect of prepared serum containing Ligusticum chuanxiong on the proliferation of HSCs was detected by MTT assay; the protein levels of CB1,FAK,and p-Akt were analyzed by Western blot. RESULTS Compared to the normal control group,24 hours treatment of PDGF-BB promoted the proliferation of HSCs significantly and increased the protein levels of CB1,FAK,and p-Akt; serums containing Ligusticum chuanxiong,colchicine and AM251 could distinctly inhibit the above effects in HSCs. After adding AM251,the inhibitory effect of serum containing Ligusticum chuanxiong was more apparent and showed a dose-dependent manner.CONCLUSION The serum containing Ligusticum chuanxiong plays the role in the prevention and treatment of hepatic fibrosis by inhibiting the proliferation of HSCs through CB1 related signaling pathway.
出处
《中成药》
CAS
CSCD
北大核心
2015年第7期1397-1401,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金预研项目(NSFYY-2011)
关键词
川芎含药血清
肝纤维化
肝星状细胞
大麻素受体1相关信号通路
黏着斑激酶
磷酸化丝氨酸/苏氨酸蛋白激酶
serum containing Ligusticum chuanxiong
hepatic fibrosis
hepatic stellate cells
cannabinoid receptor 1（CB1） related signaling pathway
focal adhesion kinase（FAK）
extracellular regulated protein kinase（p-Akt）
