摘要
目的:探讨微小RNA-155(mi R-155)对急性脑梗死患者外周CD4+CD25+调节性T淋巴细胞(Treg)的调节作用,从而了解mi R-155在急性脑梗死发病中的作用机制。方法:选取南华大学附属南华医院神经内科和重症监护室急性脑梗死患者60例(轻度20例、中度20例、重度20例)及20例正常对照组。分别采集各组的外周静脉血,采用流式细胞术检测CD4+CD25+Treg细胞的表达;实时荧光定量聚合酶联反应(RT-PCR)检测mi R-155、Foxp3 m RNA的表达;酶联免疫吸附试验(ELISA)检测白细胞介素-10(IL-10)的水平。结果 :急性脑梗死组患者外周血Treg表达率和mi R-155、Foxp3 m RNA的表达以及IL-10水平均高于正常对照组(P<0.01);并且随着临床神经功能缺损程度评分增加而升高,重中轻度组之间两两比较差异均有统计学意义(均P<0.01);死亡组各项指标显著高于生存组(均P<0.01)。mi R-155的表达与Treg和Foxp3 m RNA的表达水平均呈正相关。结论:mi R-155参与对Treg细胞增殖的调节,在急性脑梗死免疫失衡中发挥一定的作用。
Objective To investigate the adjustment of miRNA-155 on CD4＋ CD25＋ Treg regulative T cell in peripheral blood in patients with acute cerebral infarction （ACI） and its pathogenesis. Methods Sixty patients with ACI were divided into three groups according to clinical neurological deficit score. Twenty healthy volunteers were enrolled into the control group. The expression levels of plasma miR-155 mRNA and Foxp3 mRNA were detected by real-time quantitative PCR（qRT-PCR）. IL-10 levels in plasma were detected by ELISA. Results Expression of miR-155, Treg, Foxp3 mRNA and levels of IL-10 were significantly increased in patients with ACI compared with normal control group, with statistical differences; Expression of miR-155, Treg, Foxp3 mRNA and levels of IL-10 were gradually increased. The values showed significant statistical difference among the mild, moderate and severe ACI groups （P 〈 0.01 ）. Among the patients,the levels of miR-155, Treg, Foxp3 mRNA and levels of IL-10 in the survival group were obviously lower than those in the non （P 〈 0.05 or P 〈 0.01 ）. There was a positive correlation between miR-155 and Treg, Foxp3 mRNA （P 〈 0.01）. Conclusion This study suggests that miR-155 is involved in the cell proliferation regulation of CD4＋ CD25＋ Treg cells, and plays some role in the immunological dissonance with ACI.
出处
《实用医学杂志》
CAS
北大核心
2015年第11期1771-1774,共4页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:13C0706
81471310)
湖南省自然科学基金资助项目(编号:08JJ3036)
衡阳市科技局资助项目(编号:2012KJ63)
