摘要
为扩大甘蓝型油菜保持系与恢复系间的遗传差异,以波里马细胞质雄性不育系的白菜型亲本保持系Ageva、甘蓝型亲本保持系105B及相应不育系105A、8个新型甘蓝型保持系B1~B8（105B×Ageva的种间杂种回交后代）及相应不育系A1~A8、4个甘蓝型恢复系R1~R4为材料,利用分子标记分析保持系与恢复系材料间的遗传差异,用R1~R4与甘蓝型保持系进行NCⅡ双列杂交,测定其杂种表现,并对甘蓝型保持系相应不育系进行花瓣张开度和结实性调查。结果表明：保持系B1、B2、B3、B4、B5和B8与恢复系R1~R4的遗传距离均大于105B与这些恢复系的遗传距离;新型甘蓝型保持系与恢复系所配组合中,有22个组合（占总组合数的68.8%）的单株产量均高于甘蓝型亲本保持系105B与恢复系R1~R4所配杂交组合的单株产量。新型不育系A1、A3的花瓣张开度和结实性与105A接近,存在花瓣张开度大、结实性较好的新不育系材料。结论认为,导入白菜型油菜遗传成分的新甘蓝型油菜保持系应用潜力大,表现在保持系和恢复系的遗传差异大,所配组合产量高,所对应的不育系结实性好。
To broaden the variability of Brassica napus,8 new maintainer lines of B. napus were developed from interspecies hybrids of Ageva（Brassica rapa,maintainer line）and 105B（B. napus,maintainer line）. Ge-netic differences among 8 new maintainer lines B1 - B8（with corresponding sterile lines A1 - A8）,105B and 4 re-storer lines R1 - R4（B. napus）were estimated by SSR and AFLP techniques. To understand the hybrid perform-ance and combining ability,NCⅡdiallel crosses were implemented between R1 - R4 and B1 - B8,as well as 105B. Results showed that genetic distances between 6（B1,B2,B3,B4,B5 and B8）new maintainer lines and 4 restorer lines（R1 - R4）were larger than those between 105B and the corresponding restorer lines（R1 - R4）. A-mong 32 combinations between B1 - B8 and R1 - R4,22 had higher yield per plant than those between 105B and R1 - R4. A1 and A3 had larger petal - opening angles and higher seed - bearing index for making hybrids,which were much closer to 105A. In summary,heterosis of new B. napus maintainer lines had high yield potential inclu-ding broad genetic differences,higher heterosis and plant yield.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2015年第3期262-268,共7页
Chinese Journal of Oil Crop Sciences
基金
国家863计划(2011AA10A104)
国家油菜产业技术体系(CARS-13)
关键词
甘蓝型油菜
白菜型油菜
种间导入系
花瓣张开度
结实指数
Brassica napus
Brassica rapa
Introgression lines
Petal - opening angle
Seed - bearing index
