摘要
通过构建Cot-1 DNA文库以及利用荧光原位杂交技术,从赖草(Leymus secalinus(Georgi)Tzvel.)中克隆获得一个在染色体多个部位具有点状杂交信号的重复序列。进一步对该序列在赖草基因组进行克隆和分析表明该序列为一个具有90 bp的重复单元(p Ls-90),并在基因组中呈串联状排列。利用p Ls-90对赖草进行分子核型分析,结果表明:p Ls-90在染色体的着丝粒、近着丝粒、臂间以及近端粒区均有分布,而且在每对染色体上信号分布模式不尽相同,结合染色体臂比可以清楚地对赖草的14对(28条)染色体进行识别。p Ls-90不仅可作为赖草种质鉴定和利用中染色体识别的理想标记,也可作为研究小麦族不同物种染色体组进化的有力工具。
A repeat sequence producing multiple hybridization signals on chromosomes was obtained by constructing a Cot-1 DNA library and screening with a technique of fluorescence in situ hybridization（ FISH） in Leymus secalinus（ Georgi） Tzvel.. Further cloning and sequence analysis revealed that the sequence was tandem arranged in the genome with a repeated motif of 90 bp. The repetitive sequence was named as p Ls-90. Karyotyping with p Ls-90 revealed that each chromosome of L. secalinus could be well identified by the different FISH patterns with multiple hybridizations on centromeric,subcentromeric,interstitial,or subtelomeric ＂regions. p Ls-90 not only could be an ideal chromosome maker for Leymus germplasm identification and utilization but also a powerful tool for studying the genome evolution of different species in Triticeae.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2015年第4期823-827,共5页
Journal of Plant Genetic Resources
基金
青海省科技厅科技促进新农村建设计划项目(2013-N-515)
中科院"西部之光"联合学者人才培养计划项目
关键词
赖草
Cot-1
DNA
荧光原位杂交
重复序列
染色体标记
Leymus secalinus
Cot-1 DNA
FISH（fluorescence in situ hybridization）
repeat sequence
chromosome marker
