摘要
为全面评价生姜质量,通过GC-MS和HPLC法分别对不同产地生姜样品中挥发油和姜辣素进行比较分析。结果从9种生姜挥发油中分别鉴定了24~28种化学成分,占挥发油总量的85.31%~93.67%,主要为β-水芹烯(9.96%~20.15%)、莰烯(3.54%~14.53%)、柠檬醛(6.52%~12.49%)、β-柠檬醛(3.96%~7.44%)、姜烯(13.81%~19.74%)、姜黄烯(tr^5.52%)、β-倍半水芹烯(5.08%~6.22%)和β-红没药烯(2.63%~3.34%)等单萜和倍半萜类活性成分。9种生姜样品中6-姜辣素含量在0.067%~0.391%之间,均达到了现行中国药典不低于0.05%的规定。可见不同产地生姜主要活性成分含量差异较大。故以生姜的主要特征性有效成分姜烯、姜黄烯与6-姜酚共同作为其质量评价指标,将更加科学合理。
In order to evaluate the quality of ginger more comprehensively,comparative analysis of the main active constituents of ginger samples collected from different origins were performed by GC-MS and HPLC methods proposed in this work. The ginger volatile oils were extracted by water distillation method and analyzed by GC-MS. Then the chemical constituents were identified by searching NIST02 MS database and semi-quantified by peak area normalization method.Gingerols were extracted by methanol and then determined by HPLC method. The obtained results showed that 24 to 28 compounds in the volatile oils of 9 ginger samples were identified by GC-MS,which accounted for 85. 31% to 93. 67%of total essential oils,respectively. β-Phellandrene（ 9. 96% ~ 20. 15%）,camphene（ 3. 54% ~ 14. 53%）,citral（ 6. 52% ~ 12. 49%）,β-citral（ 3. 96% ~ 7. 44%）,zingiberene（ 13. 81% ~ 19. 74%）,curcumene（ tr ~ 5. 52%）,β-sesquiphellandrene（ 5. 08% ~ 6. 22%） and β-bisabolene（ 2. 63% ~ 3. 34%） were the eight main active constituents.As for non-volatile components,the contents of 6-gingerol in the 9 ginger samples were in the range of 0. 067% to0. 391%,respectively,higher than 0. 05% specified in the current Chinese pharmacopeia. But there existed obvious differences in main active constituents and their amounts among the 9 ginger samples. Thus,to specify three characteristic effective components including zingiberene,curcumene and 6-gingerol instead of one constituent 6-gingerol as the quality evaluation compounds of ginger should be much more comprehensive.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第6期1016-1021,共6页
Natural Product Research and Development
基金
国家自然科学基金(81173533)
湖南省教育厅重点项目(11A017)
