摘要
采用营养液培养法,研究了外源Ca(NO3)2对Na Cl胁迫下黄瓜幼苗生长和生理指标的影响。结果表明:60mmol·L-1Na Cl胁迫显著抑制了幼苗形态生长和生物量积累,叶片光合色素和抗坏血酸含量显著降低,质膜透性、丙二醛、脯氨酸和可溶性蛋白含量显著增加;不同浓度Ca(NO3)2处理,均使Na Cl胁迫下叶片光合色素和抗坏血酸含量显著增加,质膜透性和MDA含量显著降低,脯氨酸和可溶性蛋白含量进一步增加,幼苗生长各指标不同程度提高,其中4 mmol·L-1Ca(NO3)2处理的各项指标均优于2 mmol·L-1和6 mmol·L-1Ca(NO3)2处理。说明外源Ca(NO3)2可通过促进叶片光合色素合成,增强细胞抗氧化能力和渗透调节能力,减轻胁迫造成的膜脂过氧化伤害,缓解Na Cl胁迫对黄瓜幼苗生长的抑制,且以4mmol·L-1Ca(NO3)2处理的缓解效果最好。
The experiment was carried out to study the effect of exogenous Ca（ NO3）2on the growth and physiological indicators of cucumber seedlings under Na Cl stress in nutrient solution culture. The results showed that 60 mmol·L^- 1Na Cl stress significantly inhibited the morphological indicators and biomass accumulation of seedlings,the contents of photosynthetic pigments and ascorbic acid were significantly decreased,the membrane permeability,the contents of MDA,proline and soluble protein were significantly increased. Under salt stress,the contents of photosynthetic pigments and ascorbic acid of seedlings were significantly increased,membrane permeability and MDA content were significantly reduced,the contents of proline and soluble protein were furtherly increased,all growth indicators were improved in different extent by supplying different concentrations Ca（ NO3）2. The performance of all indicators under 4 mmol·L^- 1Ca（ NO3）2treatment were superior to 2 mmol·L^- 1 and 6 mmol·L^- 1Ca（ NO3）2treatments. That research illustrated that exogenous Ca（ NO3）2reduced the lipid peroxidation caused by Na Cl stress,alleviated the inhibition of seedling growth under Na Cl stress by regulating the synthesis of photosynthetic pigments in leaves,and enhancing the ability of antioxidation and osmotic adjustment,and the mitigation effect of 4 mmol·L^- 1 Ca（ NO3）2treatment was best.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2014年第6期22-27,共6页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
山西省留学回国人员科研资助项目(2011050)
山西省高校科技研究开发项目(20121107)
山西大同大学博士科研资助项目(2008-B-28)
山西大同大学研究所科研资助项目(2009-Y-14)
关键词
黄瓜
幼苗生长
NACL胁迫
外源钙
缓解效应
Cucumber
seedling growth
Na Cl stress
exogenous calcium
mitigation effect
