摘要
从食源中筛选出一株具有广谱抗菌活性的蜡样芽孢杆菌,为新抗菌肽的开发利用奠定基础。本实验是采用16S rDNA基因序列分析进一步鉴定具有抑菌活性的食源筛选菌株(命名为BC1),并采用琼脂扩散法测定该菌株发酵液的抑菌谱。结果表明:BC1菌株16S rDNA基因序列分析结合生理生化特征鉴定为芽孢杆菌属的蜡样芽孢杆菌;该菌株发酵液抑菌谱较宽,对金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞菌等常见病原菌具有抑制作用。
Screening the Bacillus cereus from food sources,which has broad- spectrum antimicrobial activity,so as to lay the foundation for the development and utilization of new antibacterial peptide. This experiment adapt the 16 S r DNA gene sequence analysis to further identify the strains with antibacterial activity screening from food source（ named as BC1）,and determine antibacterial spectrum from the fermentation broth via the agar punch diffusion method. The results showed that combining with physiological and biochemical characterization,BC1 was identified as Bacillus cereus of the Bacillus by 16 S r DNA gene sequence analysis. Antibacterial spectrum of the fermentation broth is wide,and have inhibitory effect on pathogenic bacteria,such as Staphylococcus aureus,Staphylococcus epidermidis and Pseudomonas aeruginosa etc.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2014年第6期1-5,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自然基金项目(2012MS1144)
