摘要
目的 探讨白细胞介素（interleukin,IL）-6单核苷酸基因多态性与中国东北地区汉族人群偏头痛及其亚型之间的相关性.方法 收集2012年3月至2013年6月辽宁省人民医院确诊的偏头痛发作期的患者252例,均为中国东北地区汉族人群.同时收集同期的健康体检者及辽宁省人民医院任职的健康职工270名,采用病例对照研究的方法,应用聚合酶链式反应技术及基因测序技术检测偏头痛组[252例;包括无先兆偏头痛（M0）组（168例）和有先兆偏头痛组（84例）;男性组（109例）和女性组（143例）]与对照组[270名;包括男性组（116名）和女性组（154名）]及二者的亚组中IL-6-572G/C（rs1800796）位点的基因型及等位基因的频率分布.结果 IL-6-572G/C位点C/C基因型和C等位基因频率在偏头痛组与对照组间的分布差异均有统计学意义[32.54％ （82/252）与19.63％ （53/270）,x2=12.061,P =0.002;55.56％ （280/504）与45.37％ （245/540）,x2=10.818,P=0.001];C/C基因型和C等位基因频率在偏头痛组与对照组的亚组女性组间进行比较,其分布差异仍有统计学意义[21.83％ （55/252）与12.59％（34/270）,x2=10.187,P=0.006;33.73％（170/504）与26.48％ （143/540）,x2=10.073,P=0.002].MO组与对照组的C/C基因型和C等位基因频率分布差异具有统计学意义[24.60％ （62/252）与19.63％ （53/270）,x2=18.172,P=0.000;39.88％ （201/504）与45.37％ （245/540）,x2=17.307,P=0.000].对该位点的C/C基因型频率分布差异进行条件Logistic回归校正后,在偏头痛组与对照组中差异仍有统计学意义（OR=22.861,95％ CI5.218 ～27.098,P=0.023）.结论 IL-6-572G/C位点基因多态性与中国东北地区汉族人群偏头痛发作相关,C/C基因型提高了中国东北地区汉族人群偏头痛的易患性.
Objective To investigate the associations of a single-nucleotide polymorphisms （SNPs） in the interleukin （IL）-6 gene with the migraine of a Han Chinese northeastern population.Methods We collected 252 patients of the migraine attack,who were diagnosed at People＇ s Hospital of Liaoning Province from March 2012 to June 2013,all being Han nationality in the northeast of China.At the same time,we collected 270 healthy people over the same period who had health examination or were healthy workers of People＇ s Hospital of Liaoning Province.We used polymerase chain reaction and gene sequencing to analyze the genotype and allele frequencies of the-572G/C （rs1800796） IL-6 polymorphisms between migraine cases （n =252;including migraine without aura （MO） group （n =168） and migraine with aura group （n =84）;the male group （n =109） and the female group （n =143）） and control group （n =270;the male group （n =116） and the female group （n =152））,as well as analyzed the genotype and allele frequencies distribution between the subgroups.Results We found higher frequencies of the distribution of the C/C genotype and the C allele of the-572G/C polymorphism in migraine cases than in control group （32.54% （82/252） vs 19.63% （53/270）,χ2 =12.061,P =0.002;55.56% （280/504） vs 45.37% （245/540）,χ2 =10.818,P =0.001）,higher distribution frequencies of the C/C genotype and the C allele in female group （21.83% （55/252） vs 12.59% （34/270）,χ2 =10.187,P =0.006;33.73% （170/504） vs 26.48% （143/540）,χ2 =10.073,P =0.002）.Between MO group and controls,these distribution frequencies had statistically significant difference （24.60% （62/252） vs 19.63% （53/270）,χ2 =18.172,P=0.000;39.88% （201/504） vs 45.37% （245/540）,χ2 =17.307,P=0.000）.After correction by conditional Logistic regression,the frequency distribution difference of the C/C genotype between the migraine cases and controls remained statistically significant （OR =22.861,95% CI 5.21
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2015年第6期498-502,共5页
Chinese Journal of Neurology
基金
沈阳市科学技术计划项目（F11-264-1-40）
关键词
偏头痛
白细胞介素6
多态性
单核苷酸
疾病遗传易感性
Migraine
Interleukin-6
Polymorphism,single nucleotide
Genetic predisposition to disease
