摘要
目的探讨鞘氨醇激酶1(Sphk1)在乳腺癌中的表达情况及其与临床病理特征及E-钙黏蛋白(E-cadherin)表达的关系。方法收集2003年9月至2006年5月重庆市肿瘤研究所乳腺外科手术切除的乳腺组织标本171例,包括乳腺纤维腺瘤20例和浸润性导管癌151例。通过免疫组织化学方法检测所有乳腺组织标本中Sphk1的表达,并分析其与乳腺癌临床病理特征及E-cadherin表达的关系。计数资料采用χ2检验,等级资料采用秩和检验。结果 Sphk1在114例乳腺癌组织中表达阳性,总阳性率为75.50%(114/151),而在乳腺纤维腺瘤组织中均表达阴性(0/20),两组差异有统计学意义(χ2=45.298,P=0.000)。Sphk1表达与乳腺癌淋巴结转移状态(Z=-6.122,P=0.000)、ER(χ2=4.478,P=0.034)及HER-2表达(χ2=7.313,P=0.013)有关,而与患者年龄(χ2=2.791,P=0.095)、月经状况(χ2=0.010,P=0.919)、肿瘤直径(Z=-0.249,P=0.804)、PR表达(χ2=0.740,P=0.390)无关。在全部浸润性导管癌患者中,E-cadherin与Sphk1表达不同的患者其淋巴结转移状态差异有统计学意义(χ2=17.187,P=0.001)。E-cadherin(-)且Sphk1(+)的患者淋巴结阳性率为85.71%(18/21),Ecadherin(+)且Sphk1(-)的淋巴结阳性率为30.77%(8/26),两者差异具有统计学意义(χ2=14.189,P<0.008)。结论 Sphk1可能在乳腺癌的发生和转移过程中发挥作用,有望作为评估乳腺癌生物学行为的指标。
Objective To investigate the expression of sphingosine kinase 1 （Sphkl） and its correlation with clinicopathological characteristics and with E-cadherin expression in breast cancer. Methods We collected 171 cases of breast tissue specimens resected in Department of Breast Surgery, Chongqing Cancer Institute from September 2003 to May 2006, including 20 cases of breast fibroadenoma and 151 cases of invasive ductal carcinoma. Immunohistochemistry was used to detect Sphkl and E-cadherin expression in all breast tissue specimens. The correlations of Sphkl expression with clinicopathological characteristics and E-cadhefin expression were analyzed. The count data were processed using χ2 test, rank data using rank sum test. Results Sphkl was positive in 114 cases of breast cancer （75.50% ,114/151 ）, negative in all 20 cases of breast fibroadenoma; the difference was significant （ χ2 = 45. 298, P = 0. 000） ; Sphkl expression was significantly associated with lymph node metastasis（ Z = -6. 122, P = 0. 000 ）, ER （ χ2 = 4. 478, P = 0. 034 ） and HER-2 expression （χ2 = 7. 313, P = 0. 013）, not correlated with the patient＇ age （ χ2 = 2. 791, P = 0. 095 ）, menopausal status （ χ2 = 0. 010, P = 0. 919）, tumor diameter （Z=-0. 249, P=0. 804）or PR expression（χ2 =0. 740,P=0. 390）. In all patients with invasive ductal carcinoma, the patients with different E-cadherin and Sphkl expression status showed a significant difference in lymph node metastasis （χ2 = 17. 187, P = 0. 001 ）. The positive rate of lymph node metastasis was 85.71% （ 18/21 ） in the patients with E-cadherin （-） and Sphkl （＋） , 30. 77% （8/26） in the patients with E- cadherin （＋） and Sphkl （ - ）, indicating a significant difference （ χ2 = 14. 189, P 〈 0. 008 ）. Conclusion Sphkl may play an important role in the development and metastasis of breast cancer, which can be a useful indicator to predict the biological behaviors of breast cancer.
出处
《中华乳腺病杂志（电子版）》
CAS
CSCD
2015年第2期21-24,共4页
Chinese Journal of Breast Disease(Electronic Edition)
基金
国家自然科学基金资助项目(81072156)
重庆市科技计划项目(cstc2013jcsf10027)
重庆市沙坪坝区社会发展领域科技项目(cstc2013jcsf0143)
重庆市卫计委科技计划项目(2012-2-505)
