摘要
[目的]研究MTDH在胰腺癌中的表达及其与钙黏蛋白(E-cadherin)、肿瘤微血管密度的关系及其临床意义。[方法]应用免疫组织化学SP法检测MTDH、E-cadherin、CD31在60例胰腺癌及其癌旁正常胰腺组织中的表达;并用抗CD31抗体标记微血管,计数微血管密度。应用即时荧光定量PCR检测10例胰腺癌及其癌旁正常胰腺组织中MTDH m RNA的表达水平。[结果]MTDH在胰腺癌组织中的表达率显著高于癌旁胰腺组织中的表达(P<0.05);胰腺癌组织MTDH的表达与患者临床分期(P=0.014)、淋巴结转移(P=0.002)和远处转移(P=0.006)相关;E-cadherin的表达与患者临床分期(P=0.002)、淋巴结转移(P=0.023)和组织学分级(P=0.027)相关。MTDH在胰腺癌组织中的表达与E-cadherin表达负相关(P<0.05),与MVD表达呈正相关(P<0.05)。[结论]MTDH在胰腺癌中高表达,其表达水平与肿瘤的进展和转移相关。MTDH蛋白的高表达可能通过影响肿瘤E-cadherin的表达和肿瘤微血管生成来促进胰腺癌的转移。
[Purpose] To investigate the expression of MTDH in pancreatic carcinoma and its correlations with E-cadherin and microvascular density. [Methods] Immunohistochemistry was used to examine the expression of MTDH,E-cadherin and CD31 in 60 cases of pancreatic cancer and corresponding cancer adjacent tissues. With CD31 antibody as an endothelial marker, the expression level of MTDH m RNA in 10 samples of pancreatic cancer and corresponding cancer adjacent tissues was detected by real-time PCR. [Results] Expression of MTDH in pancreatic cancer tissues was higher than that in cancer adjacent tissues（P〈0.05）. The expression of MTDH related to clinical stage（P =0.014）,lymph node metastasis（P =0.002）and distant metastasis（P =0.006）. The expression of E-cadherin related to clinical stage（P=0.002）,lymph node metastasis（P=0.023）and histological grade（P=0.027）. MTDH expression was positively correlated with MVD（P〈0.05） and negatively correlated with E-cadherin（P〈0.05） in pancreatic carcinoma tissues. [Conclusions] The expression of MTDH is up-regulated in pancreatic cancer,and it might relate to the progression of pancreatic cancer. MTDH might promote the progression of pancreatic carcinoma through influencing the expression of E-cadherin and promoting tumor microvessel generation.
出处
《肿瘤学杂志》
CAS
2015年第6期475-481,共7页
Journal of Chinese Oncology
基金
浙江省医药卫生一般研究计划(2013KYB228)
杭州市余杭区重大科研项目(余科【2012】68号2012-5医学)
关键词
胰腺肿瘤
肿瘤转移
异黏蛋白
pancreatic neoplasms
tumor metastasis
MTDH
