摘要
目的探讨小细胞肺癌（SCLC）患者外周血循环肿瘤细胞（CTC）的检测及其临床意义。方法选择2011年8月至2014年8月我科收治的80例小细胞肺癌患者作为观察组，收集同期本院的50例肺良性病变患者作为对照组。分别抽取外周血7．5ml，采用密度梯度离心法及qRT—PCR法检NA,细胞肺癌患者化疗2周期前后及对照组外周血中细胞角蛋白-19（CK-19）阳性的CTC的变化情况，同时分析其与临床特征及化疗疗效的关系。结果观察组中56例检测到CTC，阳性率70％，对照组未检测到CTC，差异有统计学意义（P〈0．05）。CTC阳性表达情况与SCLC患者的病理分期及复发转移相关（P〈0．05），与患者的性别、年龄、是否吸烟以及肿瘤的分化程度无相关性（P〉0．05）。观察组中化疗前CTC表达56例，化疗2周期后CTC表达24例，差异有统计学意义（P〈0．05）。标准方案化疗2周期后，CTC数目评价疗效与实体瘤疗效评价标准（RECIST）评价疗效两者比较，差异无统计学意义（χ^2=0．002，P〉0．05）。相关分析显示，CTC表达与化疗疗效呈负相关（r=-0．025，P=0．041）。结论CTC阳性表达与SCLC患者的病理分期和复发转移相关，与化疗疗效呈负相关，CTC的表达情况可以预测小细胞肺癌患者的化疗疗效及预后。
Objective To investigate the detection of tumor cells in peripheral circulation in patients with small cell lung cancer （SCLC） and its clinical significance. Methods 80 patients with SCLC treated in the Department of Oncology at Yongzhou Central Hospital from August, 2011 to August, 2014 were selected as an observation group and 50 patients with benign lung disease a control group. 7.5 ml peripheral blood was collected from the patients respectively. Density gradient centrifugation method and Quantitative Real-time Reverse Transcription-PCR method were used to detect the CTC expression with cytokeratin-19 positive in the two groups before and two cycles after the chemotherapy. Then the relationship between clinical features and chemical effect was analyzed. Results CTC expression was detected in 56 cases in the observation group, with a positive rate of 70%, and in no one in the control group, with a statistical difference between the two groups （P〈0.05）. CTC expression correlated with clinical stages and recurrence （P〈0.05）, but not with gender, age, organization type, smoking, and degree of differentiation （P〉0.05）. Before the chemotherapy, CTC expression was detected in 56 cases; after two cycles chemotherapy, CTC expression was detected in 24 cases; with a statistical difference （P〈0.05）. After two cycles chemotherapy, there were no statistical differences in chemotherapy effects between the evaluations of CTC numbers and Response Evaluation Criteria in Solid Tumors （χ^2=0.002,P〉0.05）. CTC expression correlated with the clinical efficacy （r=-0.025, P=0.041）. Conclusions The positive rate of CTC correlates with the clinical stages and recurrence and negatively with chemotherapy effect. The positive rate of CTC can predict the chemotherapy effects in SCLC.
出处
《国际医药卫生导报》
2015年第13期1840-1843,共4页
International Medicine and Health Guidance News
关键词
小细胞肺癌
循环肿瘤细胞
qRT—PCR法
化疗疗效
Small cell lung cancer
Tumor cells in peripheral circulation
Quantitative Real-time Reverse Transcription-PCR
Chemotherapy effect
