摘要
目的探讨佛甲草(SLT)提取物对S180荷瘤小鼠的抗肿瘤生长、外周血白细胞分类、血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)、一氧化氮(NO)水平的影响。方法采用小鼠右前肢腋部皮下接种S180细胞建立移植瘤模型,观察SLT提取物4、8g/kg体质量连续给药14d。观察小鼠体质量变化,计算肿瘤、胸腺及脾脏指数,检测小鼠外周血中白细胞分类,测定小鼠血清SOD、GSH-Px的活性及MDA、NO的水平。结果经SLT治疗后,S180荷瘤小鼠体质量明显高于环磷酰胺(CTX)组,肿瘤指数明显低于模型组和CTX组,差异均有统计学意义(P<0.05),且能有效的调节荷瘤小鼠的胸腺指数及脾脏指数;外周血中性粒细胞数/白细胞总数(GR%)和单核细胞/白细胞总数(MO%)明显低于模型组,差异均有统计学意义(P<0.05),而淋巴细胞/白细胞总数(LY%)明显高于模型组(P<0.05),血清SOD、GSH-Px活性明显高于模型组(P<0.05),血清MDA水平明显低于模型组和CTX组(P<0.05),血清中NO水平明显高于模型组和CTX组(P<0.05)。结论SLT对S180荷瘤小鼠有抗肿瘤作用,其抗肿瘤机制与抑制氧化应激和调节肿瘤免疫有关。
Objective To observe the antitumor activity of sedum lineare thunb（SLT） and it＇s affection on antioxidation and tumor immunology in S180-bearing mice. Methods S180-bearing mice model were established by subcutaneously inoculating S180 ascitic tumor into the right armpit. From the second day,the mice were treated with normal saline,cyelophosphamide （50 g/kg） or two doses of SLT（4,8 g/kg） through intragastric administration, and the process extent 14 d. After the last administration, the mice body weight and the index of tumor, spleen and thymus were calculated, classification of white blood cells in peripheral blood was tested,and the activity of SOD and GSH-Px as well as the content of MDA and NO in serum were measured. Results The body weight of mice treatmented with SLT was dramaticlly higher than those of the CTX （P〈 0.05）. Compare with the model group, SLT groups showed decreases in tumors index （P〈 0.05 ）, spleen index （P〈0.05）, percentage of peripheral blood neutro- phil （P〈0.05） and monocyte （P〈0.05） , and increases in thymus index （P〈0.05） and percentage of lymphocyte （P〈0.05）. The activity of SOD and GSH-Px and NO level in the SLT groups mice＇s serum were significantly higher than those of the model group （P〈0.05） ,while the content of MDA in the SLT groups micet s serum was markedly lower than those of the model and CTX groups（P〈0.05）. Conclusion SLT has antitumor activity in S180-bearing mice bysuppressing oxidative stress and modulating tumor immune.
出处
《重庆医学》
CAS
北大核心
2015年第19期2613-2615,共3页
Chongqing medicine
基金
江西省卫生厅科技项目(20141117)
关键词
佛甲草
氧化性应激
肿瘤免疫
抗肿瘤作用
sedum lineare
oxidative stress
tumor immune
antitumor activity
