摘要
目的:探讨金凤颗粒对痛风性关节炎兔关节软骨MMP-1/TIMP-1表达的影响,明确治疗痛风性关节炎作用机制。方法:采用乙胺丁醇、腺嘌呤联合灌胃+MSU关节腔注射法复制家兔痛风性关节炎模型。成模后,随机分为模型组,金凤颗粒高、中、低剂量组(19.6、98、4.9 g/kg),别嘌醇组(0.028 g/kg),痛风定组(0.448 g/kg),另设空白组。连续7 d给药。末次给药2 h后,取各组兔的关节软骨组织,观察其病理形态学改变;并采用免疫细胞化学技术检测其MMP-1、TIMP-1表达,计算MMP-1/TIMP-1比值。结果:金凤颗粒可明显减轻兔关节软骨的炎性细胞浸润、充血、肿胀和坏死,可显著抑制MMP-1的表达(P<0.05)、促进TIMP-1的活化(P<0.01),并显著降低MMP-1/TIMP-1比值(P<0.05)。结论:金凤颗粒可有效抑制痛风性关节炎的病理损害,其作用机制可能与其抑制MMP-1的表达、上调TIMP-1的表达,调节MMP-1/TIMP-1平衡有关,进而发挥抗炎作用。
Objective： To explore the effect of Jinfeng particles on the expression of MMP-1 /TIMP-1 in cartilage of gout arthritis rabbit＇s articular,to explicit treatment mechanism of gouty arthritis. Methods： Perfusion of ethambutol and adenine,inject MSU into joint cavity of rabbits to copy the model of gouty arthritis. After successful mode,were randomly divided into model group,Jinfeng granules in high,medium and low dose group（ 19. 6,9. 8,4. 9 g / kg）,allopurinol group（ 0. 028 g / kg）,Tongfengding group（ 0. 448 g / kg）,another blank group. Administered for 7 days. 2 h after the last administration,were taken and painted articular cartilage tissue,to observe the changes of the pathological morphology; and the use of immune cell detection of the expression of TIMP-1,MMP-1,MMP-1 /TIMP-1 ratio calculation. Results： Jinfeng granule could obviously reduce the articular cartilage of rats with inflammatory cells infiltration,hyperemia,swelling and necrosis,could significantly inhibit the expression of MMP-1（ P〈0. 05）,stimulate TIMP-1 activation（ P〈0. 01）,and significantly reduce the ratio of MMP-1 / TIMP-1（ P〈0. 05）. Conclusion： Jinfeng particles can effectively inhibit the pathological damage of gouty arthritis,upside the expression of TIMP-1 and its mechanism may be related to the inhibition of MMP-1,regulated the balance of MMP-1 / TIMP-1,and then exert anti-inflammatory effects.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第6期774-777,784,共5页
Chinese Journal of Immunology
基金
2014年湖南省研究生科研创新项目(No.CX2014B350)
企业委托课题:中药六类新药金凤颗粒的临床前研究
