摘要
为了探明高粱幼苗耐冷生理机制,以高粱耐冷和冷敏材料幼苗为试验材料,利用智能光照培养箱设置低温条件,分析了低温和常温条件下高粱耐冷和冷敏材料的丙二醛(MDA)含量、超氧化物岐化酶(SOD)活性和过氧化物酶(POD)活性。结果表明,低温胁迫后,两类材料的MDA含量、SOD活性、POD活性都出现了增高的趋势,耐冷材料MDA含量的增加幅度低于冷敏材料,耐冷材料的SOD活性、POD活性的增加幅度则显著高于冷敏材料,说明材料的耐冷与否,与低温胁迫后MDA含量、SOD活性、POD活性的增加量有关,MDA含量的增加幅度愈小材料愈耐冷,SOD活性、POD活性的增加幅度愈大材料愈耐冷。
In order to understand the chilling tolerance physiological mechanisms of sorghum seedling, the author analyzed the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and peroxidase (POD) activity under the condition of low temperature and room temperature with a chilling tolerant variety and a chilling susceptible variety as the test materials. The results showed that MDA content, SOD activity and POD activity of two types of materials showed an increasing trend after low temperature stress. The increase of the MDA of chilling tolerant variety was below that of the chilling susceptible variety, while the increases of SOD activity and POD activity of chilling tolerant variety were above that of the chilling susceptible variety. It proved that whether variety was resistant to cold or not was related to the increase of MDA content, SOD activity and POD activity. Smaller increase of MDA content in material could result in more tolerance to cold; while larger increase of SOD activity and POD activity could result in more tolerance to cold.
出处
《中国农学通报》
2015年第18期74-79,共6页
Chinese Agricultural Science Bulletin
基金
山西省自然科学基金项目"高粱耐冷性鉴定及耐冷生理机制研究"(2013011030-2)
山西省农业科学院攻关项目"高粱耐冷机制研究"(2012ygg03)
山西省农业科学院攻关项目"提高高粱耐冷性研究"(YGG1403)