摘要
目的:建立流式细胞术筛查血小板抗体方法应用于临床检测。方法:对78例需要进行血小板抗体检测的患者作流式细胞筛查术检测,同时采用经典酶联免疫吸附试验(PAKPLUS试剂盒)做对照,比较2种方法的实验结果。结果:流式细胞术的血小板抗体检出率为35.90%(28/78),PAKPLUS的检出率为25.65%(20/78),2种方法阳性率差异无统计学意义(P>0.05);经MAIPA技术鉴定,流式细胞术筛查血小板抗体为阳性的患者中,有10例含抗HLA抗体,有1例含抗HPA-5b抗体,有9例为自身抗体。结论:成功建立了流式细胞术筛查血小板抗体并应用于临床检测;该技术拥有快速、易于操作的特点,且能实现程序化、标准化检测,值得推广应用。
Objective：To establish platelet antibody screening using Flow Cytometry（FCM）and apply it to the clinic.Method：The platelet antibodies in 78 patients were detected by FCM and PAKPLUS,respectively.The results between two different techniques were compared.Result：In 78 patients,28 were positive in FCM（35.90%）,20 were positive in PAKPLUS（25.65%）,and there was no significant difference between two methods（P〉0.05）.After identification by MAIPA,10 patients were anti-HLA,one patient was anti-HPA-5b,9patients were autoantibody.Conclusion：We established FCM technique to screen platelet antibody successfully.FCM may become the standard platelet antibody screening method as a possible alternative to be recommended.
出处
《临床血液学杂志（输血与检验）》
CAS
2015年第3期459-461,共3页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
基金
广西科学研究与技术开发计划项目(主席资金
No:08160-05)
南宁市科学研究与技术开发计划项目(科技惠民重大科技专项
No:20133141)
广西自然科学基金项目(No:2012GXNSFBA053127)
广西自然科学基金项目(No:2013GXNSFBA019206)
