摘要
以红菇子实体为原料,采用超声波方法提取水溶性多糖,从中分离纯化了3个组分（R1、R2、R3）,研究其对多种敏感菌的抑菌活性。结果表明：组分R1具有明显的抑菌活性,能够抑制细菌,其中对革兰氏阴性菌抑菌活性强于革兰氏阳性菌,对酵母和霉菌无抑制效果;而R2无抑菌活性,R3抑菌活性弱。组分R1对各供试菌株的最小抑制质量浓度分别为金黄色葡萄球菌2.5mg·mL^-1,藤黄微球菌10mg·mL^-1,蜡样芽孢杆菌5mg·mL^-1,枯草芽孢杆菌10mg·mL^-1,大肠杆菌1.0mg·mL^-1,铜绿假单胞菌1.25mg·mL^-1,痢疾志贺氏菌1.25mg·mL-1;具有较好的耐热性和较宽的pH适用范围,在80℃处理30min和pH6-10都能维持很好的抑菌活性;其抑菌作用方式是杀菌,对菌体无溶菌作用。
The water-soluble polysaccharide was extracted using ultrasound method from fruit body of Russula sp. ,which was colloct- ed from wild resources in eastern Guangdong province. Three components were sperated and purified from crude polysaccharide using chromatography. The component RI exhibited strong antibacterial activity towards Gram-negative bacteria than Gram-positive bacteri- a,and had no activity to yeast and mold. Fraction R2 didn＇t show any antibacterial actility and fraction R3 showed weak actility. The minimum inhibitory concentration for each of the tested strains were Staphylococcus aureus 2. 5 mg ·mL^-1, Micrococcus luteus 10 mg·mL^-1 , Bacillus cereus 5 mg·mL^-1, Bacillus subtilis 10 mg·mL^-1 , Escherichia coli 1.0 mg·mL^-1, Pseudomonas aeruginosa 1.25 mg·mL^-1, Shigella dysentery 1.25 mg·mL^-1. The polysaccharide has good heat resistance and a wide pH range, it can maintain a good antibacterial activity at 80 ℃ for 30 min and pH 6 - 10. The Antimicrobial mode of action of action of the polysac- charides is sterilization, no cell lysis.
出处
《福建林业科技》
2015年第2期98-102,共5页
Journal of Fujian Forestry Science and Technology
基金
广东省潮州市科技计划项目(2010N09)
韩山师范学院青年基金资助(LQ200808)
关键词
红菇
子实体多糖
抑菌作用
Russula sp.
polysaccharide of fruit body
antibacterial activity
