摘要
目的:探讨黄芪多糖对脂肪细胞释放肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的抑制作用及机制.方法由3T3-L1细胞诱导分化成熟的脂肪细胞随机分为实验组和对照组.实验组脂肪细胞以0.1g·L-1黄芪多糖培养基干预,对照组脂肪细胞普通培养基培养.ELISA法测两组脂肪细胞及培养基中TNF-α、IL-6蛋白的含量,及逆转录-聚合酶链反应检测两组脂肪细胞TNF-α蛋白和IL-6蛋白mRNA水平.结果实验组脂肪细胞及培养基中TNF-α、IL-6蛋白的含量低于对照组(P=0.000),而且实验组脂肪细胞TNF-α蛋白和IL-6蛋白mRNA水平低于对照组(P=0.000).结论:黄芪多糖可抑制脂肪细胞释放炎症细胞因子,控制脂肪组织的慢性炎症反应.
Objective： To explore the inhibition of astragalus polysaccharides（APS） on secretion of tumor necrosis factor alpha（TNF-a） and interleukin-6 （IL-6）. Methods： 3T3-L1 preadipocytes was cultured and induced into mature adipocytes. These adipocytes were then divided into experiment group and control group. Adipocytes were treated with 0. lg L APS in experiment group,but control group adipocytes were cultured normally. The TNF-a and IL-6 contents in the cultured superna- tant and these adipocytes two groups were determined by ELISA method, and the two proteins mRNA were adipocytes by re- verse transcription-polymerase chain reaction（RT-PCR）. All datas were analysed by one-samples test. Results The TNF-a and IL-6 contents in the cultured supernatant and adipocytes in experiment group were significantly lower than control group, and the levels of two proteins＇ mRNA in experiment group were also significantly lower than control group. These results show statistical significance（P〈0.05）. Conclusion： APS has the inhivitory effect on secretion inflammatory cytokines from ad- ipocytes, so control chronic inflammation of adipose tissue.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2015年第3期121-124,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省科技厅资助项目(072300450370)
2013年度新乡医学院研究生科研创新支持计划项目
关键词
黄芪多糖
脂肪细胞
肿瘤坏死因子-Α
白介素-6
astragalus polysaccharides
adipocytes
tumor necrosis factor alpha
interleukin-6
