摘要
目的：采用RP-HPLC-ELSD法动态测定不同生长年限的加拿大原产地西洋参叶中皂苷的含量。方法：RP-HPLC-ELSD法、主成分分析法和聚类分析法。结果：1年生西洋参叶总皂苷含量较其它生长年限的低,2~5年生西洋参叶总皂苷含量积累较多;人参皂苷Rg1、Re、Rb2、Rb1、Rc和Rg3以及拟人参皂苷F11和RT5是西洋参叶特征性的皂苷成分。按聚类分析,将1年生的叶聚为第1类,2~5年生的叶聚为第2类,且2~5年生叶的总皂苷含量趋于平稳。结论：每年9~10月割取西洋参地上部分（茎叶）,作为人参皂苷提取的植物来源。
Objective： To determine the dynamic accumulation of ginsenoside in P. quinquefolium L. leaf from differ- ent grown years and different collection months in Canada. Methods： A RP-HPLC-ELSD, principal component analysis and cluster analysis were adopted. Results： The contents of one-year-old were lower than others. Principal component analysis showed that Rgl, Re, Rb2, Rb1, Rc and Rg3 were regarded as the characteristic ginsenosides of P. quirtque- folium L. leaf. Cluster analysis showed that samples of one-year-old was the first major categories, the others was the second categories. Conclusion： It was suggested that stem of Panax quinquefolium L. should be collected for extracts of ginsenoside before stem and leaf withered of Sept. to Oct. every year.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2015年第5期247-253,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家科技部重大新药创制科技重大专项(2011ZX09307-002-01)
国家科技支撑计划项目(2012BAI27B07)
国家自然科学基金项目(No.81173653)
