摘要
目的探讨非小细胞肺癌患者抑癌基因CDH13及DAPK基因CpG岛甲基化情况及其临床意义。方法采用甲基化特异性-PCR方法检测40例非小细胞肺癌患者(肺癌组)和30例肺良性病变患者(肺良性病变组)的抑癌基因CDH13及DAPK的Cp G岛甲基化的情况。结果肺癌组织CDH13、DAPK基因CpG岛甲基化率分别为47.5%(19/40)、40.0%(16/40),癌旁组织分别为27.5%(11/40)、30.0%(12/40),肺良性病变组织均为0。癌组织、癌旁组织CDH13、DAPK的CpG岛甲基化率均明显高于良性病变组(P<0.05),但癌组织与癌旁组织间比较,差异无统计学意义(P>0.05)。鳞癌与腺癌组织CDH13、DAPK的CpG岛甲基化率比较,差异均无统计学意义(P>0.05)。结论非小细胞肺癌组织存在CDH13、DAPK的CpG岛甲基化,其与非小细胞肺癌发生发展有着密切关系。
Objective To explore CpG island methylation and clinical significance of CDH13 and DAPK genes in non-small cell lung carcinoma patients.Methods Methylation specific PCR was used to detect the CpG island methylation status of CDH13 and DAPK genes among 40 patients with non-small cell lung carcinoma（lung cancer group） and 30 patients with benign lung diseases（benign lung disease group）R.esults The incidences of CpG island methylation of CDH 13 and DAPK genes were 47.5%（19/40） and 40.0%（16/40） respectively in the lung cancer group,27.5%（11/40） and 30.0%（12/40） respectively in the para-carcinoma group, and 0 in the benign lung disease group.The incidences of CpG island methylation of CDH13 and DAPK genes were significantly higher in the lung cancer group and para-carcinoma group in contrast with those in the benign lung disease group（P〈0.05）,which showed no significant difference between lung cancer group and para-carcinoma group（P〉0.05）.There was no significant difference in the incidences of CpG island methylation of CDH13 and DAPK genes between lung squamous carcinoma tissues and lung adenocarcinoma tissues（P〉0.05）.Conclusion The CpG island methylation of CDH13and DAPK genes can be observed in the non-small cell lung cancer tissues, and it is associated with the development of non-small cell lung carcinoma.
出处
《广西医学》
CAS
2015年第4期449-451,共3页
Guangxi Medical Journal
基金
广西科学研究与技术开发计划项目(桂科攻1355055-3-19)
