摘要
目的:探讨肌细胞增强因子2A(Myocyte Enhancer Factor 2a,MEF2A)基因单核苷酸多态性(SNP)与早发冠心病血瘀证的相关性。方法:从MEF2A基因选取2个SNP位点rs2033547和rs34851361,建立基于飞行时间质谱分析技术(TOF-MS)的方法,对收集的早发CHD血瘀证组21例,早发CHD非血瘀组30例,非"早发冠心病"血瘀组25例与健康对照组20例,检测各SNP位点的等位基因和基因分型,对比各组等位基因和各基因型频率,并比较各临床资料。结果:rs2033547、rs34851361都有A和T两种等位基因,包括AA、TT、AT 3种基因型;MEF2A基因多态性位点rs2033547的A等位基因在早发CHD血瘀证组,早发CHD非血瘀证组和非"早发CHD"血瘀证组均高于健康对照组,但差异尚无统计学意义(P>0.05),这可能与样本量不足有关。而MEF2A基因多态性位点rs34851361基因型和等位基因在4组间相似,无统计学意义(P>0.05),两两比较亦无统计学意义(P>0.05)。结论:MEF2A基因多态性位点rs2033547和rs34851361非早发冠心病的易感基因位点。
Objective：To investigate the relationship between the Myocyte Enhancer Factor. 2a（ MEF2A ）gene single nucleo-tide polymorphism and the blood stasis syndrome of premature coronary heart disease （PCHD）. Methods：Choose two SNPs rs2033547and rs34851361 in the MEF2A gene,detecting the SNPs of 21 cases in the group of blood- stasis syndrome of PCHD, 30 cases in the group of non - blood - stasis syndrome of PCHD ,25 cases in the group of blood - stasis syndrome of non - PCHD and 20 cases in the control group base on the technology of the time of flight mass spectrometry technique（ TOF MS） and compa- ring the allele and genotype frequencies of each group. Results：There are two alleles A. T and three genetype AA. TT. AT in rs2033547, rs34851361. The A allele of rs2033547 in MEF2A gene in the group of blood - stasis syndrome of PCHD, non - blood stasis syndrome of PCHD and blood -stasis syndrome of non -PCHD is higher than control group, but it has no statistical sig- nificance（ P 〉 0.05 ）. Maybe the number of case is not enough. In the four groups,the polymorphism rs34851361 in MEF2A gene is similar. It has no statistical significance（ P 〉 0. 05 ） among four groups. Comparing the two, it also has no statistical significance （P 〉 0. 05 ）. Conclusion：The MEF2A gene SNP namely rs2033547 and rs34851361 is not associated with PCHD.
出处
《辽宁中医杂志》
CAS
北大核心
2015年第6期1155-1158,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81001518
81373550
81202647)
霍英东教育基金会资助(131042)
中国博士后科学基金(2012M521526
2013T60771)
中医诊断学国家重点学科研究生创新基金(2013ZYZD28)
关键词
早发冠心病
MEF2A基因
单核苷酸多态性
飞行时间质谱
premature coronary heart disease
MEF2A gene
single nucleotide polymorphisms
time - of - flight mass spec-trometry
