摘要
为了研究高相对分子质量多环芳烃(PAHs)芘的生物共代谢降解,考察了低相对分子质量PAHs芴和菲的加入对芘产生的影响,并采用GC-MS测定了生物降解后代谢产物的组成。结果表明,单一PAHs的生物降解中,芴在培养的第5d已被完全降解,生成5种代谢产物,菲在第7d降解率达到98.93%,生成10种代谢产物,芘在第9d时降解率仅为65.73%,生成较多代谢产物,其中6种可基本定性;3种PAHs混合降解时,芴、菲和芘分别在第3d、5d和8d完全被除去,共产生8种代谢产物,其中芘在第8d时只产生了3种代谢产物。芴和菲的存在不仅促进了芘的完全快速降解,而且能够促进芘代谢产物的去除,芘的存在也促进了芴和菲的降解和代谢产物的去除。
In order to study the co-metabolism biodegradation of the polycyclic aromatic hydrocarbons （PAHs） with high relative molecular mass, an influence of the addition of fluorene and phenanthrene, the PAHs with low relative molecular mass, on pyrene biodegradation was investigated and the composition of the biodegradation metabolites was determined by GC-MS. The results showed that in the biodegradation of single PAHs, fluorene was completely degraded throughout 5 d incubation to generate five metabolites, the degradation rate of phenanthrene reached 98.93% to generate ten metainbolites after 7 d incubation and only 65.73% of pyrene was degraded throughout 9 d incubation to generate more metabolites, six of which could be qualitative. In the degradation of mixture of fluorene, phenanthrene and pyrene, they were completely removed after 3 d, 5 d and 8 d incubation, respectively, and a total of eight metabolites were produced, and only three metabolites were produced from pyrene after 8 d incubation. Therefore, fluorene and phenanthrene not only promoted the degradation of pyrene rapidly and completely, but also promoted the removal of the metabolites of pyrene, meanwhile, the presence of pyrene also contributed to the degradation of fluorene and phenanthrene and the removal of their metabolites.
出处
《石油学报（石油加工）》
EI
CAS
CSCD
北大核心
2015年第3期720-725,共6页
Acta Petrolei Sinica(Petroleum Processing Section)
基金
辽宁省教育厅科学研究项目(L2014148)资助
关键词
多环芳烃
共代谢
生物降解
polycyclic aromatic hydrocarbons
co-metabolism biodegradation
